![Western Blot: NOL12 Antibody [NBP2-19560]](https://resources.rndsystems.com/images/products/NOL12-Antibody-Western-Blot-NBP2-19560-img0002.jpg "Western Blot: NOL12 Antibody [NBP2-19560]")
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human NOL12. The exact sequence is proprietary.
Localization
Nucleus; nucleolus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NOL12 Antibody
Western Blot: NOL12 Antibody [NBP2-19560]
Western Blot: NOL12 Antibody [NBP2-19560] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.![Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560]](https://resources.rndsystems.com/images/products/NOL12-Antibody-Immunohistochemistry-Paraffin-NBP2-19560-img0007.jpg "Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560]")
Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560]
Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560] - NOL12 antibody detects NOL12 protein at nucleous on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. NOL12 antibody dilution: 1:500.![Western Blot: NOL12 Antibody [NBP2-19560]](https://resources.rndsystems.com/images/products/NOL12-Antibody-Western-Blot-NBP2-19560-img0001.jpg "Western Blot: NOL12 Antibody [NBP2-19560]")
Western Blot: NOL12 Antibody [NBP2-19560]
Western Blot: NOL12 Antibody [NBP2-19560] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE gel, diluted at 1:1000.![Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560]](https://resources.rndsystems.com/images/products/NOL12-Antibody-Immunohistochemistry-Paraffin-NBP2-19560-img0005.jpg "Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560]")
Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560]
Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560] - NOL12 antibody detects NOL12 protein at nucleous on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. NOL12 antibody dilution: 1:500.![Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560]](https://resources.rndsystems.com/images/products/NOL12-Antibody-Immunohistochemistry-Paraffin-NBP2-19560-img0006.jpg "Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560]")
Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560]
Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560] - NOL12 antibody detects NOL12 protein at nucleous on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. NOL12 antibody dilution: 1:500.Applications for NOL12 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NOL12
Alternate Names
dJ37E16.7, FLJ34609, MGC3731, Nop25, nucleolar protein 12, RRP17
Gene Symbol
NOL12
UniProt
Additional NOL12 Products
Product Documents for NOL12 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NOL12 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Customer Reviews for NOL12 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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