NOP56 Antibody (CL2603) - BSA Free

Novus Biologicals | Catalog # NBP2-36778

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2603

Format

BSA Free
View all NOP56 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from O00567, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NOP56 Antibody (CL2603) - BSA Free

Western Blot: NOP56 Antibody (CL2603) [NBP2-36778]

Western Blot: NOP56 Antibody (CL2603) [NBP2-36778]

Western Blot: NOP56 Antibody (CL2603) [NBP2-36778] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NOP56 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778]

Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778]

Immunocytochemistry/Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778] - Staining in MCF7 cell line with Anti-NOP56 monoclonal antibody, showing nucleoli-specific staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778]

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778]

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human breast cancer shows strong nucleolar positivity in tumor cells.
Western Blot: NOP56 Antibody (CL2603) [NBP2-36778]

Western Blot: NOP56 Antibody (CL2603) [NBP2-36778]

Western Blot: NOP56 Antibody (CL2603) [NBP2-36778] - Lane 1: Marker [kDa]. Lane 2:Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778]

Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778]

Immunocytochemistry/Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778] - Staining in HeLa cell line showing nucleoli-specific staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778]

Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778]

Immunocytochemistry/Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human cell line A431 showing nucleoli-specific staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778]

Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778]

Immunocytochemistry/Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778] - Staining in U2OS cell line with Anti-NOP56 monoclonal antibody, showing nucleoli-specific staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778]

Immunocytochemistry/ Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778]

Immunocytochemistry/Immunofluorescence: NOP56 Antibody (CL2603) [NBP2-36778] - Staining in U251 cell line showing nucleoli-specific staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778]

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778]

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human prostate cancer shows strong nucleolar positivity in glandular cells.
Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778]

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778]

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human fallopian tube shows strong nucleolar immunoreactivity in glandular and connective tissue cell
Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778]

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778]

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human kidney shows strong nucleolar positivity in renal tubules.
Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778]

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778]

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human cervix shows strong nucleolar immunoreactivity in the epithelial cells.
Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778]

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778]

Immunohistochemistry: NOP56 Antibody (CL2603) [NBP2-36778] - Staining of human liver shows strong nucleolar positivity in hepatocytes.
NOP56 Antibody (CL2603) - BSA Free

NOP56 (CL2603) in A431 Human Cell Line.

NOP56 (CL2603) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti-NOP56 (CL2603) Protein A Purified Monoclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NBP2-36778) (green) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for NOP56 Antibody (CL2603) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NOP56

Nop56p is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Nop56p is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. The protein encoded by this gene is similar in sequence to Nop56p and is also found in the nucleolus. Multiple transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of most of them have not been determined.

Alternate Names

NOL5A, NOP56 ribonucleoprotein homolog (yeast), nucleolar protein 56, Nucleolar protein 5A, nucleolar protein 5A (56kD with KKE/D repeat), nucleolar protein 5A (56kDa with KKE/D repeat)

Gene Symbol

NOP56

UniProt

O00567 (Human)

Additional NOP56 Products

Product Documents for NOP56 Antibody (CL2603) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NOP56 Antibody (CL2603) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NOP56 Antibody (CL2603) - BSA Free

There are currently no reviews for this product. Be the first to review NOP56 Antibody (CL2603) - BSA Free and earn rewards!

Have you used NOP56 Antibody (CL2603) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies