NOP56 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13666

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NOP56 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EDPSISFSKPKKKKSFSKEELMSSDLEETAGSTS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NOP56 Antibody - BSA Free

Western Blot: NOP56 Antibody [NBP2-13666]

Western Blot: NOP56 Antibody [NBP2-13666]

Western Blot: NOP56 Antibody [NBP2-13666] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-NOP56 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: NOP56 Antibody [NBP2-13666]

Immunocytochemistry/ Immunofluorescence: NOP56 Antibody [NBP2-13666]

Immunocytochemistry/Immunofluorescence: NOP56 Antibody [NBP2-13666] - Staining of human cell line U-2 OS shows localization to nucleoli fibrillar center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NOP56 Antibody [NBP2-13666]

Immunohistochemistry-Paraffin: NOP56 Antibody [NBP2-13666]

Immunohistochemistry-Paraffin: NOP56 Antibody [NBP2-13666] - Human kidney shows weak positivity in nucleoli in cells in tubules.
Immunohistochemistry-Paraffin: NOP56 Antibody [NBP2-13666]

Immunohistochemistry-Paraffin: NOP56 Antibody [NBP2-13666]

Immunohistochemistry-Paraffin: NOP56 Antibody [NBP2-13666] - Human pancreas shows weak postivity in nucleoli in exocrine glandular cells.
Immunohistochemistry-Paraffin: NOP56 Antibody [NBP2-13666]

Immunohistochemistry-Paraffin: NOP56 Antibody [NBP2-13666]

Immunohistochemistry-Paraffin: NOP56 Antibody [NBP2-13666] - Staining of human fallopian tube shows moderate postivity in nucleoli in glandular cells.
Immunohistochemistry-Paraffin: NOP56 Antibody [NBP2-13666]

Immunohistochemistry-Paraffin: NOP56 Antibody [NBP2-13666]

Immunohistochemistry-Paraffin: NOP56 Antibody [NBP2-13666] - Staining of human cerebral cortex shows strong positivity in nucleoli in neurons.

Applications for NOP56 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NOP56

Nop56p is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Nop56p is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. The protein encoded by this gene is similar in sequence to Nop56p and is also found in the nucleolus. Multiple transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of most of them have not been determined.

Alternate Names

NOL5A, NOP56 ribonucleoprotein homolog (yeast), nucleolar protein 56, Nucleolar protein 5A, nucleolar protein 5A (56kD with KKE/D repeat), nucleolar protein 5A (56kDa with KKE/D repeat)

Entrez Gene IDs

10528 (Human)

Gene Symbol

NOP56

Additional NOP56 Products

Product Documents for NOP56 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NOP56 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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