NPM1 Antibody (7H10B9)

Novus Biologicals | Catalog # NBP1-47354

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Monkey

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7H10B9
View all NPM1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human NPM (2-265) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NPM1 Antibody (7H10B9)

Western Blot: NPM1 Antibody (7H10B9)BSA Free [NBP1-47354]

Western Blot: NPM1 Antibody (7H10B9)BSA Free [NBP1-47354]

Western Blot: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis using NPM mouse mAb against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: NPM1 Antibody (7H10B9) - BSA Free [NBP1-47354]

Immunocytochemistry/ Immunofluorescence: NPM1 Antibody (7H10B9) - BSA Free [NBP1-47354]

Immunocytochemistry/Immunofluorescence: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis of Hela (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Immunohistochemistry-Paraffin: NPM1 Antibody (7H10B9) - BSA Free [NBP1-47354]

Immunohistochemistry-Paraffin: NPM1 Antibody (7H10B9) - BSA Free [NBP1-47354]

Immunohistochemistry-Paraffin: NPM1 Antibody (7H10B9) [NBP1-47354] - Analysis of human liver carcinoma tissues, showing nuclear localization using NPM mouse mAb with DAB staining.

Applications for NPM1 Antibody (7H10B9)

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NPM1

Nucleophosmin (NPM) is a multi-functional protein that is involved in many cellular activities including cell growth, proliferation and transformation. It is frequently overexpressed in solid tumors, and has recently been found to be mutated and aberrantly localized in the cytoplasm of leukaemic blasts in patients with acute myeloid leukemia (AML).

NMP antibodies are useful tools as tumor markers in cancer research.

Long Name

Nucleophosmin/Nucleolar Phosphoprotein B23

Alternate Names

B23, Nucleophosmin, Numatrin

Entrez Gene IDs

4869 (Human)

Gene Symbol

NPM1

Additional NPM1 Products

Product Documents for NPM1 Antibody (7H10B9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NPM1 Antibody (7H10B9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Acute Myeloid Leukemia
p53 Pathway

Citations for NPM1 Antibody (7H10B9)

Customer Reviews for NPM1 Antibody (7H10B9)

There are currently no reviews for this product. Be the first to review NPM1 Antibody (7H10B9) and earn rewards!

Have you used NPM1 Antibody (7H10B9)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies