NPM1 Antibody - BSA Free

Novus Biologicals | Catalog # NB110-61646

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NPM1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal region of human mutant Nucleophosmin (within residues 250-294). [Accession# AAW67752]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30214626).

Localization

Nucleophosmin typically resides in the nucleus. However, in patients with acute myelogenous leukemia (AML) it mutates and localizes to the cytoplasm.

Specificity

This antibody is specific for the mutant form of Nucleophosmin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NPM1 Antibody - BSA Free

Western Blot: NPM1 AntibodyBSA Free [NB110-61646]

Western Blot: NPM1 AntibodyBSA Free [NB110-61646]

Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.
Immunocytochemistry/ Immunofluorescence: NPM1 Antibody - BSA Free [NB110-61646]

Immunocytochemistry/ Immunofluorescence: NPM1 Antibody - BSA Free [NB110-61646]

Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in HL-60 cells (negative control).
Immunocytochemistry/ Immunofluorescence: NPM1 Antibody - BSA Free [NB110-61646]

Immunocytochemistry/ Immunofluorescence: NPM1 Antibody - BSA Free [NB110-61646]

Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in OCI-AML3 cells.

Applications for NPM1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

1:50 - 1:100

Immunohistochemistry-Paraffin

1:50 - 1:100

Immunoprecipitation

1:50

Western Blot

1:500
Application Notes
In WB a band can be seen at ~37 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 2 reviews rated 3 using NB110-61646 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NPM1

Nucleophosmin (NPM; also known as B23, numatrin or NO38) is a highly conserved phosphoprotein that belongs to nucleoplasmin/nucleophosmin family of nuclear chaperones. Expressed ubiquitously with nucleolar localization, NPM shuttles between nucleoli and cytoplasm during cell cycle progression. It binds ribosome to drive ribosome nuclear export and has been associated with nucleolar ribonucleoprotein structures. Chaperonin are the proteins that provide favourable conditions for the correct folding of other proteins for preventing aggregation; and NPM has the ability to acts as chaperonin for the core histones H3, H2B and H4 proteins. NPM stimulates APEX1 endonuclease activity on A/P double-stranded DNA while inhibit the same on A/P single-stranded RNA, and regulate APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA as well as the removal of oxidized rRNA molecules. BRCA2 helps NPM in regulating centrosome duplication, whereas, PLK2 mediated phosphorylation controls the centriole duplication. NPM is implicated in diverse cellular processes including cell proliferation, and regulation of tumor suppressors such as p53/TP53 and ARF.

Long Name

Nucleophosmin/Nucleolar Phosphoprotein B23

Alternate Names

B23, Nucleophosmin, Numatrin

Gene Symbol

NPM1

Additional NPM1 Products

Product Documents for NPM1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NPM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Acute Myeloid Leukemia
p53 Pathway

Citations for NPM1 Antibody - BSA Free

Customer Reviews for NPM1 Antibody - BSA Free (2)

3 out of 5
2 Customer Ratings
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Customer Images

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  • NPM1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 03/16/2018
    There was strong signal, but the signal was strong in the both the nucleus and the cytoplasm. 1:100 dilution was used, so perhaps using more diluted antibody would help.
    NPM1 Antibody - BSA Free NB110-61646
  • NPM1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human lymphocytes
    Species: Human
    Verified Customer | Posted 01/18/2018
    1:500 16 hours 4C in 5% milk-TBS/T 1 minute exposure, 25ug protein.
    NPM1 Antibody - BSA Free NB110-61646

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Protocols

View specific protocols for NPM1 Antibody - BSA Free (NB110-61646):

NPM1 Antibody:
Western Blot

1. Run 50 ug of cytoplasmic fraction into each lane of an 12% SDS-PAGE.

2. Block the membrane.

3. Dilute the primary anti-mNPM1 (NB110-61646) in 1% NFDM and incubate for 2 hours at room temperature.

4. Wash the membrane.

5. Dilute the sedondary anti-rabbit IgG, conjugated to HRP in 1% NFDM and incubate for 1 hour at room temperature.

6. Develop the blot.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for NPM1 Antibody - BSA Free

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  • Q: Does this antibody require antigen retrieval? If so, what antigen retrieval method do you recommend?

    A: For the validation of our Nucleophosmin antibody (NB110-61646), we did not use any customized protocol and in our routine protocol, we use Sodium Citrate buffer pH 6.0 based antigen retrieval method. Please note that formalin fixation as well as paraffin embedding crosslinks the proteins which decreases staining signal by causing conformational changes on the antigens. The inclusion/conditions of antigen retrieval depends primarily upon for how long the samples were in the fixative solution. If the fixation time for samples was more than 4 hours, we would recommend performing Sodium Citrate buffer ph 6.0 based antigen retrieval.

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