Nrf2 Antibody (2G7) - Azide and BSA Free

Novus Biologicals | Catalog # H00004780-M02

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2G7

Format

Azide and BSA Free
View all Nrf2 Primary Antibodies »

Product Specifications

Immunogen

NFE2L2 (AAH11558, 71 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FAQLQLDEETGEFLPIQPAQHIQSETSGSANYSQVAHIPKSDALYFDDCMQLLAQTFPFVDDNEVSSATFQSLVPDIPGHIESPVFIATNQAQSPETSVA

Reactivity Notes

Mouse reactivity reported from customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

nuclear factor (erythroid-derived 2)-like 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Nrf2 Antibody (2G7) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Nrf2 Antibody (2G7) [H00004780-M02]

Immunocytochemistry/ Immunofluorescence: Nrf2 Antibody (2G7) [H00004780-M02]

Immunocytochemistry/Immunofluorescence: Nrf2 Antibody (2G7) [H00004780-M02] - Analysis of monoclonal antibody to NFE2L2 on HeLa cell. Antibody concentration 10 ug/ml.
ELISA: Nrf2 Antibody (2G7) [H00004780-M02]

ELISA: Nrf2 Antibody (2G7) [H00004780-M02]

ELISA: Nrf2 Antibody (2G7) [H00004780-M02] - Detection limit for recombinant GST tagged NFE2L2 is approximately 3ng/ml as a capture antibody.
Nrf2 Antibody (2G7)

Western Blot: Nrf2 Antibody (2G7) [H00004780-M02] -

h00004780-m02_mouse-monoclonal-nrf2-antibody-2g7-20920231412572.jpg
Nrf2 Antibody (2G7)

Western Blot: Nrf2 Antibody (2G7) [H00004780-M02] -

Western Blot: Nrf2 Antibody (2G7) [H00004780-M02] - EMPA treatment attenuated cardiomyocyte overproduction of reactive oxygen species (ROS) induced by TAC. (A) Left, Mitosox staining of cardiomyocytes in sham, sham + EMPA, TAC & TAC + EMPA groups & Right, quantitative results. (B) Representative blots of NRF-2 & HO-1, & quantitative results. (C) The relative mRNA levels of genes related to endogenous antioxidants. Results are expressed as mean ± SEM, n = 5–7, *p < 0.05 vs. corresponding sham group, †p < 0.05 vs. corresponding TAC vehicle group. One-way ANOVA & Tukey post hoc test. EMPA, empagliflozin; SEM, standard error of the mean; TAC, transverse aortic constriction; NRF-2, the nuclear factor erythroid 2–related factor 2; HO-1, heme oxygenase-1; GCLM, glutamate-cysteine ligase modifier subunit; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35647080), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Nrf2 Antibody (2G7) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Reviewed Applications

Read 1 review rated 5 using H00004780-M02 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Nrf2

NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM]

Long Name

NF-E2-related Factor 2

Alternate Names

NFE2L2

Entrez Gene IDs

4780 (Human)

Gene Symbol

NFE2L2

OMIM

600492 (Human)

UniProt

AAH11558 (Human)

Additional Nrf2 Products

Product Documents for Nrf2 Antibody (2G7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Nrf2 Antibody (2G7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nrf2 Antibody (2G7) - Azide and BSA Free

Customer Reviews for Nrf2 Antibody (2G7) - Azide and BSA Free (1)

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Customer Images

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  • Name: Nadine Bakkar
    Application: Western Blot
    Sample Tested: Mouse spinal cords
    Species: Mouse
    Verified Customer | Posted 08/06/2014
    Western Blot on mouse spinal cord homogenates using NRF2 antiboody (2G7)and Ms 680 (LicoR) secondary antibody.
    Nrf2 Antibody (2G7) - Azide and BSA Free H00004780-M02

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Protocols

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FAQs

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Associated Pathways

MAPK Signaling: Oxidative Stress Pathway MAPK Signaling: Oxidative Stress Pathway Thumbnail