NSDHL Antibody (20F2) - BSA Free

Novus Biologicals | Catalog # NBP3-26457

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 20F2 expressed in HEK293

Format

BSA Free
View all NSDHL Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human NSDHL [UniProt Q15738]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NSDHL Antibody (20F2) - BSA Free

NSDHL Antibody (20F2)

Immunohistochemistry: NSDHL Antibody (20F2) [NBP3-26457] -

Immunohistochemistry: NSDHL Antibody (20F2) [NBP3-26457] - Image of NSDHL Antibody (20F2) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
NSDHL Antibody (20F2)

Western Blot: NSDHL Antibody (20F2) [NBP3-26457] -

Western Blot: NSDHL Antibody (20F2) [NBP3-26457] - Positive Western Blot detected in: HepG2 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, HEK293 whole cell lysate.
All lanes: NSDHL Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 42 kDa
Observed band size: 42 kDa

Applications for NSDHL Antibody (20F2) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: NSDHL

NSDHL is encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene. [provided by RefSeq]

Alternate Names

EC 1.1.1.170, H105E3, member 1, NAD(P) dependent steroid dehydrogenase-like, SDR31E1, sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating, XAP104

Gene Symbol

NSDHL

Additional NSDHL Products

Product Documents for NSDHL Antibody (20F2) - BSA Free

Certificate of Analysis

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Product Specific Notices for NSDHL Antibody (20F2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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