NSMAF Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84735

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NSMAF Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EKVAMLTQILEFGQTPKQLFVTPHPRRITPKFKSLSQTSSYNASMADSPGEESFEDLTEESKTLAWNNITKLQLHEHYKIHKEAVTGITVSRNGSSVFTT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NSMAF Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NSMAF Antibody [NBP1-84735]

Immunocytochemistry/ Immunofluorescence: NSMAF Antibody [NBP1-84735]

Immunocytochemistry/Immunofluorescence: NSMAF Antibody [NBP1-84735] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.
NSMAF Antibody - BSA Free Immunohistochemistry-Paraffin: NSMAF Antibody - BSA Free [NBP1-84735]

Immunohistochemistry-Paraffin: NSMAF Antibody - BSA Free [NBP1-84735]

Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.
NSMAF Antibody - BSA Free Immunohistochemistry-Paraffin: NSMAF Antibody - BSA Free [NBP1-84735]

Immunohistochemistry-Paraffin: NSMAF Antibody - BSA Free [NBP1-84735]

Staining of human skeletal muscle shows moderate cytoplasmic and nuclear positivity in myocytes.
NSMAF Antibody - BSA Free Immunohistochemistry-Paraffin: NSMAF Antibody - BSA Free [NBP1-84735]

Immunohistochemistry-Paraffin: NSMAF Antibody - BSA Free [NBP1-84735]

Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
NSMAF Antibody - BSA Free Immunohistochemistry-Paraffin: NSMAF Antibody - BSA Free [NBP1-84735]

Immunohistochemistry-Paraffin: NSMAF Antibody - BSA Free [NBP1-84735]

Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
NSMAF Antibody - BSA Free Western Blot: NSMAF Antibody - BSA Free [NBP1-84735]

Western Blot: NSMAF Antibody - BSA Free [NBP1-84735]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp

Applications for NSMAF Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NSMAF

Cellular responses to tumor necrosis factor (TNF; MIM 191160) are initiated by the interaction of TNF with 2 distinct cell surface receptors that transmit signals to the cytoplasm and nucleus, leading to profound alterations in transcriptional programs. The initiation of intracellular signaling events through 1 of these receptors, the 55-kD tumor necrosis factor receptor-1 (TNFR1; MIM 191190), appears to depend on protein intermediates that interact with specific cytoplasmic domains of TNFR1 (Adam-Klages et al., 1996 [PubMed 8808629]).[supplied by OMIM]

Alternate Names

Factor associated with neutral sphingomyelinase activation, FANFactor associated with N-SMase activation, neutral sphingomyelinase (N-SMase) activation associated factor, protein FAN

Gene Symbol

NSMAF

Additional NSMAF Products

Product Documents for NSMAF Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NSMAF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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