NT-4 Antibody (OTI2E8)

Novus Biologicals | Catalog # NBP1-47897

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2E8
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Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 81-210 human NT4 (NP_006170) produced in E.coli.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Please contact Technical Support if you have any Mouse-On-Mouse questions. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens neurotrophin 4 (NTF4).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse NT-4 Antibody (OTI2E8) (NBP1-47897) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-NT-4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for NT-4 Antibody (OTI2E8)

Western Blot: NT-4 Antibody (OTI2E8) [NBP1-47897]

Western Blot: NT-4 Antibody (OTI2E8) [NBP1-47897]

Western Blot: NT-4 Antibody (2E8) [NBP1-47897] - Neurotrophin 4 antibody (2E8) at 1:5000 + Recombinant Human Neurotrophin 4.
Immunocytochemistry/ Immunofluorescence: NT-4 Antibody (OTI2E8) [NBP1-47897]

Immunocytochemistry/ Immunofluorescence: NT-4 Antibody (OTI2E8) [NBP1-47897]

Immunocytochemistry/Immunofluorescence: NT-4 Antibody (2E8) [NBP1-47897] - Staining of A549 cells using anti-NTF4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NT-4 Antibody (OTI2E8) [NBP1-47897]

Immunohistochemistry-Paraffin: NT-4 Antibody (OTI2E8) [NBP1-47897]

Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Human Kidney tissue using anti-NTF4 mouse monoclonal antibody.
Western Blot: NT-4 Antibody (OTI2E8) [NBP1-47897]

Western Blot: NT-4 Antibody (OTI2E8) [NBP1-47897]

Western Blot: NT-4 Antibody (2E8) [NBP1-47897] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NTF4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTF4.
Immunohistochemistry-Paraffin: NT-4 Antibody (OTI2E8) [NBP1-47897]

Immunohistochemistry-Paraffin: NT-4 Antibody (OTI2E8) [NBP1-47897]

Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NTF4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NT-4 Antibody (OTI2E8) [NBP1-47897]

Immunohistochemistry-Paraffin: NT-4 Antibody (OTI2E8) [NBP1-47897]

Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NTF4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NT-4 Antibody (OTI2E8) [NBP1-47897]

Immunohistochemistry-Paraffin: NT-4 Antibody (OTI2E8) [NBP1-47897]

Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NTF4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NT-4 Antibody (OTI2E8) [NBP1-47897]

Immunohistochemistry-Paraffin: NT-4 Antibody (OTI2E8) [NBP1-47897]

Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NTF4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NT-4 Antibody (OTI2E8) [NBP1-47897]

Immunohistochemistry-Paraffin: NT-4 Antibody (OTI2E8) [NBP1-47897]

Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Human colon tissue using anti-NTF4 mouse monoclonal antibody.

Applications for NT-4 Antibody (OTI2E8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NT-4

NT4 is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of NT4 is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NT4-deficient mice only show minor cellular deficits and develop normally to adulthood

Long Name

Neurotrophin 4

Alternate Names

NT-5, NT4, NTF4, NTF5

Entrez Gene IDs

4909 (Human)

Gene Symbol

NTF4

Additional NT-4 Products

Product Documents for NT-4 Antibody (OTI2E8)

Certificate of Analysis

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Product Specific Notices for NT-4 Antibody (OTI2E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NT-4 Antibody (OTI2E8)

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Protocols

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