NUCKS1 Antibody (3G10) - Azide and BSA Free

Novus Biologicals | Catalog # H00064710-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3G10

Format

Azide and BSA Free
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Product Specifications

Immunogen

NUCKS1 (NP_073568.2, 1 a.a. ~ 243 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSRPVRNRKVVDYSQFQESDDADEDYGRDSGPPTKKIRSSPREAKNKRRSGKNSQEDSEDSEDKDVKTKKDDSHSAEDSEDEKEDHKNVRQQRQAASKAASKQREMLMEDVGSEEEQEEEDEAPFQEKDSGSDEDFLMEDDDDSDYGSSKKKNKKMVKKSKPERKEKKMPKPRLKATVTPSPVKGKGKVGRPTASKASKEKTPSPKEEDEEPESPPEKKTSTSPPPEKSGDEGSEDEAPSGED

Specificity

This product is specific for Human NUCKS1 monoclonal antibody (M02), clone 3G10 [Gene ID: 64710].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for NUCKS1 Antibody (3G10) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: NUCKS1 Antibody (3G10) [H00064710-M02]

Immunocytochemistry/ Immunofluorescence: NUCKS1 Antibody (3G10) [H00064710-M02]

Immunocytochemistry/Immunofluorescence: NUCKS1 Antibody (3G10) [H00064710-M02] - Analysis of monoclonal antibody to NUCKS1 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: NUCKS1 Antibody (3G10) [H00064710-M02] - Detection limit for recombinant GST tagged NUCKS1 is 0.3 ng/ml as a capture antibody.

Applications for NUCKS1 Antibody (3G10) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml
Application Notes
Mouse monoclonal antibody raised against a full-length recombinant NUCKS1.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NUCKS1

NUCKS (nuclear ubiquitous casein and cyclin-dependent kinases substrate) is a nuclear phosphoprotein phosphorylated by Cdk1 during mitosis. It is a DNA-binding protein and bears two putative nuclear localization signals. NUCKS has been shown to localize to the cytoplasm in mitotic cells and is targeted to the nucleus during telophase. It is expressed ubiquitously and possesses features of a housekeeping gene. Alternate names for NUCKS include NUCKS1, JC7, and FLJ21480.

Alternate Names

FLJ21480, FLJ32016, FLJ38536, NUCKSnuclear ubiquitous casein and cyclin-dependent kinases substrate, nuclear casein kinase and cyclin-dependent kinase substrate 1, nuclear ubiquitous casein kinase and cyclin-dependent kinase substrate, P1, potential LAG1 interactor

Gene Symbol

NUCKS1

Additional NUCKS1 Products

Product Documents for NUCKS1 Antibody (3G10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NUCKS1 Antibody (3G10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for NUCKS1 Antibody (3G10) - Azide and BSA Free

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  • Q: If we receive a small trial aliquot of NUCKS1 antibody (3G10), cat. no H00064710-M02, we can support you with documentation (pictures) if the antibody works well. We have long experience with working on the NUCKS1 protein, including immune cytochemistry, Western immuno-blotting and immune precipitation.

    A:

    Our NUCKS1 antibody, catalog number H00064710-M02, has been validated for Western blotting and ELISA with human samples. Unfortunately we are unable to send out free samples of this product; however you may be interested in our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a 100% credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

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