Nuclear Factor Erythroid Derived 2 Antibody (JE65-92)

Western Blot: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646]

Western Blot: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646] - Western blot analysis of Nuclear Factor Erythroid Derived 2 on different lysates with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/1,000 dilution.

Lane 1: U-937 cell lysate (15 ug/Lane)
Lane 2: K-562 cell lysate (15 ug/Lane)
Lane 3: TF-1 cell lysate (15 ug/Lane)
Lane 4: HeLa cell lysate (15 ug/Lane)
Lane 5: HepG2 cell lysate (15 ug/Lane)
Lane 6: Mouse testis tissue lysate (30ug/Lane)
Lane 7: Rat testis tissue lysate (30ug/Lane)

Predicted band size: 41 kDa
Observed band size: 41 kDa

Exposure time: 1 minute 20 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32646) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646]

Immunohistochemistry: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32646) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646]

Immunocytochemistry/ Immunofluorescence: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646] - Immunocytochemistry analysis of K-562 cells labeling Nuclear Factor Erythroid Derived 2 with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.