Nucleobindin 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19601

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Nucleobindin 1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nucleobindin 1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%).

Localization

Golgi apparatus; cis-Golgi network membrane; Peripheral membrane protein; Lumenal side (By similarity); Cytoplasm (By similarity)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nucleobindin 1 Antibody - BSA Free

Western Blot: Nucleobindin 1 Antibody [NBP2-19601]

Western Blot: Nucleobindin 1 Antibody [NBP2-19601]

Western Blot: Nucleobindin 1 Antibody [NBP2-19601] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody [NBP2-19601]

Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody [NBP2-19601]

Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody [NBP2-19601] - Nucleobindin 1 antibody detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleobindin 1 protein stained by Nucleobindin 1 antibody diluted at 1:500. Red: FIS1, a mitochondria marker, stained by FIS1 antibody diluted at 1:20000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP2-19601]

Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP2-19601]

Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP2-19601] - Mouse kidney. Nucleobindin 1 antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody [NBP2-19601]

Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody [NBP2-19601]

Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody [NBP2-19601] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP2-19601]

Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP2-19601]

Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP2-19601] - Mouse kidney. Nucleobindin 1 antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Nucleobindin 1 Antibody - BSA Free

Western Blot: Nucleobindin 1 Antibody [NBP2-19601] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Nucleobindin 1 antibody [HL2530] (NBP2-19601) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for Nucleobindin 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Nucleobindin 1

This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq]

Alternate Names

DKFZp686A15286, FLJ40471, NUCCALNUC, nucleobindin 1, nucleobindin-1

Gene Symbol

NUCB1

UniProt

Q02818 (Human)

Additional Nucleobindin 1 Products

Product Documents for Nucleobindin 1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Nucleobindin 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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