Nucleoli Marker Antibody (NM95) - IHC-Prediluted
Novus Biologicals | Catalog # NBP2-45215
Key Product Details
Species Reactivity
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # NM95
Format
IHC-Prediluted
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Product Specifications
Immunogen
Nuclei of myeloid leukemia biopsy cells
Reactivity Notes
Does not react with Mouse, Rat or Bovine.
Localization
Nucleoli
Marker
Marker For Human Cells
Specificity
This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. monoclonal antibody NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Nucleoli Marker Antibody (NM95) - IHC-Prediluted
Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215]
Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215] - Staining of human colon ca.Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215]
Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Nucleoli Marker Antibody (NM95).Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215]
Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215] - Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleoli Marker Antibody (NM95).Flow Cytometry: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215] -
Flow Cytometry of Human Nucleolar Ag on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled human Nucleoli Marker Antibody (NM95) - IHC-Prediluted.Immunocytochemistry/ Immunofluorescence: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215] -
Immunofluorescence Analysis of PFA-fixed K562 cells labeling Nucleolin with Nucleoli Marker Antibody (NM95) - IHC-Prediluted followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.Flow Cytometry: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215] -
Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleoli Marker Antibody (NM95) - IHC-Prediluted followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)Flow Cytometry: Nucleoli Marker Antibody (NM95) - IHC-Prediluted [NBP2-45215] -
Flow Cytometry of Human Nucleolar Ag on 293T cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled human Nucleoli Marker Antibody (NM95) - IHC-Prediluted.Applications for Nucleoli Marker Antibody (NM95) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.25 - 0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Product Documents for Nucleoli Marker Antibody (NM95) - IHC-Prediluted
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Nucleoli Marker Antibody (NM95) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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