Nucleoli Marker Antibody (NM95)
Novus Biologicals | Catalog # NBP2-32886
Key Product Details
Species Reactivity
Applications
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Product Specifications
Immunogen
Reactivity Notes
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Marker
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Description
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Nucleoli Marker Antibody (NM95)
Immunocytochemistry/ Immunofluorescence: Nucleoli Marker Antibody (NM95) [NBP2-32886]
Immunocytochemistry/Immunofluorescence: Nucleoli Marker Antibody (NM95) [NBP2-32886] - Immunofluorescence Analysis of PFA-fixed K562 cells labeling Nucleolin with followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) [NBP2-32886]
Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) [NBP2-32886] - Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleoli Marker Antibody (NM95).Flow Cytometry: Nucleoli Marker Antibody (NM95) [NBP2-32886]
Flow Cytometry: Nucleoli Marker Antibody (NM95) [NBP2-32886] - Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleoli Marker Antibody (NM95)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) [NBP2-32886]
Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) [NBP2-32886] - Staining of human colon ca.Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) [NBP2-32886]
Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) [NBP2-32886] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Nucleoli Marker Antibody (NM95).Flow Cytometry: Nucleoli Marker Antibody (NM95) [NBP2-32886]
Flow Cytometry: Nucleoli Marker Antibody (NM95) [NBP2-32886] - Flow Cytometry of human nucleolar Ag on 293T cells Black: cells alone: Grey: isotype control: Green: AF488-labeled human Nucleolar Monoclonal antibody (NM95)Flow (Intracellular): Nucleoli Marker Antibody (NM95) [NBP2-32886] -
Flow (Intracellular): Nucleoli Marker Antibody (NM95) [NBP2-32886] - An intracellular stain was performed on Jurkat cells with p27/Kip1 Antibody (KIP1/769) NBP2-47769PE and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.Applications for Nucleoli Marker Antibody (NM95)
Flow Cytometry
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry-Frozen
Immunohistochemistry-Paraffin
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
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Product Documents for Nucleoli Marker Antibody (NM95)
Certificate of Analysis
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Product Specific Notices for Nucleoli Marker Antibody (NM95)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Nucleoli Marker Antibody (NM95)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars