Nucleolin Antibody (NCL/7014R)

Novus Biologicals | Catalog # NBP3-11037

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # NCL/7014R
View all Nucleolin Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Nucleolin protein (Uniprot: P19338)

Reactivity Notes

Does not react with Mouse, Feline, Bovine, Canine or Equine.

Localization

Nucleolus

Marker

Marker of Human Cells

Specificity

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11038)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Nucleolin Antibody (NCL/7014R)

Western Blot: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Western Blot: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Western Blot: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Western blot analysis of Jurkat cell lysate using Nucleolin antibody (NCL/7014R).
Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded cow heart stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Human tonsil stained with Nucleolin Antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Analysis of human cells administered in pig brain using Nucleolin antibody. Antibody did NOT stain porcine tissue allowing the presence of human cells to be determined. Primary antibody dilution 1:100. Image from verified customer review.
Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded human salivary gland stained with Nucleolin Antibody (NCL/7014R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Antibody (NCL/7014R).
Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Antibody (NCL/7014R).
Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded cat liver stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded horse lung stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037]

Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/7014R) [NBP3-11037] - Formalin-fixed, paraffin-embedded mouse stomach stained with Nucleolin Antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Nucleolin Antibody (NCL/7014R)

Nucleolin Antibody (NCL/7014R)

Formalin-fixed, paraffin-embedded human colon stained with Nucleolin antibody (NCL/7014R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for Nucleolin Antibody (NCL/7014R)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

1-2ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 2 reviews rated 5 using NBP3-11037 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Nucleolin

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

Alternate Names

C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Gene Symbol

NCL

UniProt

P19338 (Human)

Additional Nucleolin Products

Product Documents for Nucleolin Antibody (NCL/7014R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Nucleolin Antibody (NCL/7014R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Nucleolin Antibody (NCL/7014R) (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
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1 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Nucleolin Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: brain and spinal cord
    Species: Pig
    Verified Customer | Posted 02/01/2022
    Nucleolin 1:100 on pig brain
    We were looking for human cells that were administered in pig brain. We needed an antibody that did not stain porcine tissue. This antibody did not stain pig and helped us determine the presence of human cells.
    Nucleolin Antibody (NCL/7014R) NBP3-11037
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Nucleolin Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human tonsil
    Species: Human
    Verified Customer | Posted 06/03/2021
    Human tonsil stained with Nucleolin Antibody
    Citrate pH6 retrieval at 110C, antibody dilution 1:100/30 min RT, anti-rabbit polymer detection
    Nucleolin Antibody (NCL/7014R) NBP3-11037

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