NuMA Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NuMA Antibody [NB500-174]

Immunocytochemistry/Immunofluorescence: NuMA Antibody [NB500-174] - NuMA antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunocytochemistry/ Immunofluorescence: NuMA Antibody [NB500-174]

Immunocytochemistry/Immunofluorescence: NuMA Antibody [NB500-174] - NuMA antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunocytochemistry/ Immunofluorescence: NuMA Antibody [NB500-174]

NuMA-Antibody-Immunocytochemistry-Immunofluorescence-NB500-174-img0024.jpg

Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174]

Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal breast using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. The breast ductal/acinar epithelial cells, the myoepithelial cells and some cells in the surrounding connective tissue depicted a strong nuclear along with moderate cytoplasmic immuno-positivity for NuMA protein. The intra-lobular and the surrounding connective tissue also developed weak/potentially non-specific staining. 10X Magnification.

Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174]

Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal spleen using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. Most of the cells developed a strong nuclear along with moderate cytoplasmic immuno-positivity for NuMA protein. 10X Magnification.

Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174]

Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal skin using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. Almost all the cells in the epidermal layer and the cells in the connective tissue of dermal layer depicted very strong nuclear along with moderate cytoplasmic immuno-positivity for NuMA protein. 10X Magnification.

Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174]

Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal brain using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. The cells in the brain tissue depicted strong specific nuclear along with weak cytoplasmic immuno-positivity for NuMA protein. 10X Magnification.

Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174]

Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal kidney using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. The cells of various tubules/ducts and Bowman's capsule in the renal tissue depicted strong nuclear along with relatively weak cytoplasmic immuno-positivity for NuMA protein. 10X Magnification.

Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174]

Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal prostate using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. The prostatic alveolar glandular epithelium as well as the surrounding fibromuscular stroma cells showed a strong nuclear immuno-reactivity for NuMA protein. Almost all the cells developed some cytoplasmic staining also. 10X Magnification.

Simple Western: NuMA Antibody [NB500-174]

Simple Western: NuMA Antibody [NB500-174] - Image shows a specific band for NuMA in 0.2 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.

Western Blot: NuMA Antibody - BSA Free [NB500-174] -

NuMA, but not HSET, Aurora A kinase, or TACC3, is upregulated in cyclin D1-overexpressing cells.(A) Western blot of NuMA, TACC3, HSET, Aurora A kinase, and cyclin D1 levels in MCF10APuro (control) and MCF10ACyclin D1 cell lines. The overexpressed cyclin D1 is HA-tagged. Vinculin is shown as a loading control. (B-C) Quantification of NuMA, cyclin D1 (B), HSET, Aurora A kinase, and TACC3 levels (C) normalized to vinculin levels. Lines show mean +/- standard deviation of three independent replicates. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0296779), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: NuMA Antibody - BSA Free [NB500-174] -

NuMA localizes to minus-ends despite dynein inhibition or knockout.(A) Live images of GFP-alpha -tubulin immediately before and after k-fiber ablation at targeted sites (red ‘X’s) in a PtK2 cell in which dynein cargo-binding is inhibited by transfection of the dominant-negative p150-CC1 fragment (Quintyne and Schroer, 2002). Scale bar, 5 μm. (B) Immunofluorescence image of NuMA (magenta) and alpha -tubulin (yellow) in cell from (A), fixed after ablation. NuMA (arrowheads) localizes to new minus ends. Scale bar, 5 μm. (C) Western blot showing >65% depletion (normalized to alpha -tubulin) of dynein intermediate chain (DIC) protein at the cell population level after dynein heavy chain (DHC) depletion using inducible-Cas9 DHC-knockout HeLa cells (McKinley and Cheeseman, 2017). DIC depletion has been shown to correlate with DHC depletion (Levy and Holzbaur, 2008). Because knockout was heterogeneous across the population, dynein loss within individual cells chosen for analysis was always verified by immunofluorescence, as in (D), or by spindle phenotype. (D) Immunofluorescence images of inducible DHC-knockout HeLa cells show robust localization of NuMA at minus-ends after DHC knockout, in the absence of dynein (dynein intermediate chain; DIC). Scale bar, 5 μm. (E) Plot of mean normalized GFP-NuMA intensity and SEM (shading) over time at ablation-created minus-ends in HeLa cells with and without dynein deletion (DHC KO). Time = 0 s at the first frame following ablation (‘X’). n = 12 ablations, 7 cells for control; n = 10 ablations, 8 cells for DHC KO. (F) Time from ablation to half maximum GFP-NuMA intensity, calculated for each individual ablation (see Methods) and then averaged for data in (E). Error bars show SEM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29185983), licensed under a CC-BY license. Not internally tested by Novus Biologicals.