Nup153 Antibody (3I5V3)

Novus Biologicals | Catalog # NBP3-16136

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3I5V3 expressed in HEK293
View all Nup153 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NUP153 (P49790). MASGAGGVGGGGGGKIRTRRCHQGPIKPYQQGRQQHQGILSRVTESVKNIVPGWLQRYFNKNEDVCSCSTDTSEVPRWPENKEDHLVYADEESSNITDGR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

154 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nup153 Antibody (3I5V3)

Western Blot: Nup153 Antibody (3I5V3) [NBP3-16136]

Western Blot: Nup153 Antibody (3I5V3) [NBP3-16136]

Western Blot: Nup153 Antibody (3I5V3) [NBP3-16136] - Western blot analysis of extracts of various cell lines, using Nup153 Rabbit mAb (NBP3-16136) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Nup153 Antibody (3I5V3)

Immunohistochemistry: Nup153 Antibody (3I5V3) [NBP3-16136] -

Immunohistochemistry: Nup153 Antibody (3I5V3) [NBP3-16136] - Immunohistochemistry analysis of Nup153 in paraffin-embedded human tonsil tissue using Nup153 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Nup153 Antibody (3I5V3)

Immunohistochemistry: Nup153 Antibody (3I5V3) [NBP3-16136] -

Immunohistochemistry: Nup153 Antibody (3I5V3) [NBP3-16136] - Immunohistochemistry analysis of Nup153 in paraffin-embedded human cervix cancer tissue using Nup153 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Nup153 Antibody (3I5V3)

Immunohistochemistry: Nup153 Antibody (3I5V3) [NBP3-16136] -

Immunohistochemistry: Nup153 Antibody (3I5V3) [NBP3-16136] - Immunohistochemistry analysis of Nup153 in paraffin-embedded mouse testis tissue using Nup153 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Nup153 Antibody (3I5V3)

Immunocytochemistry/ Immunofluorescence: Nup153 Antibody (3I5V3) [Nup153] -

Immunocytochemistry/ Immunofluorescence: Nup153 Antibody (3I5V3) [Nup153] - Immunofluorescence analysis of U-2 OS cells using Nup153 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Nup153 Antibody (3I5V3)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nup153

Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex specific glycoproteins characterized by cytoplasmically oriented O linked N acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. Nup153 has three distinct domains: a N terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C terminal region containing multiple XFXFG repeats. Nup153 is a possible DNA binding subunit of the nuclear pore complex (NPC). The repeat containing domain may be involved in anchoring components of the pore complex to the pore membrane.

Alternate Names

CG4453, Dmel\CG4453, dmNup153, dNup153, HNUP153, N153, nuclear pore complex protein hnup153,153 kDa nucleoporin, nuclear pore complex protein Nup153, nucleoporin 153kD, nucleoporin 153kDa, Nucleoporin Nup153, Nup 153, NUP153, Nup153 Nucleoporin 153

Gene Symbol

NUP153

Additional Nup153 Products

Product Documents for Nup153 Antibody (3I5V3)

Certificate of Analysis

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Product Specific Notices for Nup153 Antibody (3I5V3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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