Nup153 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81725

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Nup153 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GQNREQRESGFSYPNFSLPAANGLSSGVGGGGGKMRRERTRFVASKPLEEEEMEVPVLPKISLPITSSSLPTFNFSSPEITTSSPSPINSSQALTNKVQMTSP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nup153 Antibody - BSA Free

Western Blot: Nup153 Antibody [NBP1-81725]

Western Blot: Nup153 Antibody [NBP1-81725]

Western Blot: Nup153 Antibody [NBP1-81725] - Analysis in human cell line K562.
Immunocytochemistry/ Immunofluorescence: Nup153 Antibody [NBP1-81725]

Immunocytochemistry/ Immunofluorescence: Nup153 Antibody [NBP1-81725]

Immunocytochemistry/Immunofluorescence: Nup153 Antibody [NBP1-81725] - Staining of human cell line U-251 MG shows localization to nuclear membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725]

Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725]

Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725] - Staining of human testis shows strong positivity in nuclear membrane in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725]

Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725]

Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725] - Analysis in human testis and pancreas tissues. Corresponding NUP153 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725]

Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725]

Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725] - Staining of human endometrium shows strong positivity in nuclear membrane in glandular cells.
Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725]

Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725]

Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725] - Staining of human pancreas shows weak positivity in nuclear membrane in islets of Langerhans.
Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725]

Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725]

Immunohistochemistry-Paraffin: Nup153 Antibody [NBP1-81725] - Staining of human prostate shows moderate positivity in nuclear membrane and cytoplasm in smooth muscle cells.

Applications for Nup153 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nup153

Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex specific glycoproteins characterized by cytoplasmically oriented O linked N acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. Nup153 has three distinct domains: a N terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C terminal region containing multiple XFXFG repeats. Nup153 is a possible DNA binding subunit of the nuclear pore complex (NPC). The repeat containing domain may be involved in anchoring components of the pore complex to the pore membrane.

Alternate Names

CG4453, Dmel\CG4453, dmNup153, dNup153, HNUP153, N153, nuclear pore complex protein hnup153,153 kDa nucleoporin, nuclear pore complex protein Nup153, nucleoporin 153kD, nucleoporin 153kDa, Nucleoporin Nup153, Nup 153, NUP153, Nup153 Nucleoporin 153

Gene Symbol

NUP153

Additional Nup153 Products

Product Documents for Nup153 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Nup153 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nup153 Antibody - BSA Free

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Protocols

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