NUP50 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19610

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chimpanzee (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NUP50 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NUP50. The exact sequence is proprietary.

Localization

Nucleus; nuclear pore complex; Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side (By similarity)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NUP50 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NUP50 Antibody [NBP2-19610]

Immunocytochemistry/ Immunofluorescence: NUP50 Antibody [NBP2-19610]

Immunocytochemistry/Immunofluorescence: NUP50 Antibody [NBP2-19610] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NUP50 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2500.
Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-19610]

Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-19610]

Immunohistochemistry-Paraffin: NUP50 Antibody [NBP2-19610] - Immunohistochemical analysis of paraffin-embedded Normal colon, using antibody at 1:250 dilution.
Western Blot: NUP50 Antibody [NBP2-19610]

Western Blot: NUP50 Antibody [NBP2-19610]

Western Blot: NUP50 Antibody [NBP2-19610] - 50 ug mouse testis lysate/extract 10% SDS-PAGE NUP50 antibody [C3], C-term dilution: 1:1000.
NUP50 Antibody

Western Blot: NUP50 Antibody [NBP2-19610] -

Western Blot: NUP50 Antibody [NBP2-19610] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NUP50 antibody [C3], C-term (NBP2-19610) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for NUP50 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NUP50

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

50 kDa nucleoporin, MGC39961, NPAP60, NPAP60LNucleoporin Nup50, nuclear pore complex protein Nup50, Nuclear pore-associated protein 60 kDa-like, nuclear pore-associated protein 60L, nucleoporin 50kD, nucleoporin 50kDa

Gene Symbol

NUP50

UniProt

Q9UKX7 (Human)

Additional NUP50 Products

Product Documents for NUP50 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NUP50 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for NUP50 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review NUP50 Antibody - BSA Free and earn rewards!

Have you used NUP50 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies