NUP62 Antibody (2D3) - Azide and BSA Free

Novus Biologicals | Catalog # H00023636-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2D3

Format

Azide and BSA Free
View all NUP62 Primary Antibodies »

Product Specifications

Immunogen

NUP62 (NP_714941, 423 a.a. ~ 522 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LQHADEEREKTYKLAENIDAQLKRMAQDLKDIIEHLNTSGAPADTSDPLQQICKILNAHMDSLQWIDQNSALLQRKVEEVTKVCEGRRKEQERSFRITFD

Specificity

NUP62 - nucleoporin 62kDa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for NUP62 Antibody (2D3) - Azide and BSA Free

Western Blot: NUP62 Antibody (2D3) [H00023636-M02]

Western Blot: NUP62 Antibody (2D3) [H00023636-M02]

Western Blot: Nucleoporin p62 Antibody (2D3) [H00023636-M02] - NUP62 monoclonal antibody (M02), clone 2D3. Analysis of NUP62 expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: NUP62 Antibody (2D3) [H00023636-M02]

Immunocytochemistry/ Immunofluorescence: NUP62 Antibody (2D3) [H00023636-M02]

Immunocytochemistry/Immunofluorescence: Nucleoporin p62 Antibody (2D3) [H00023636-M02] - Analysis of monoclonal antibody to NUP62 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: NUP62 Antibody (2D3) [H00023636-M02]

Western Blot: NUP62 Antibody (2D3) [H00023636-M02]

Western Blot: Nucleoporin p62 Antibody (2D3) [H00023636-M02] - NUP62 monoclonal antibody (M02), clone 2D3. Analysis of NUP62 expression in PC-12.
Western Blot: NUP62 Antibody (2D3) [H00023636-M02]

Western Blot: NUP62 Antibody (2D3) [H00023636-M02]

Western Blot: Nucleoporin p62 Antibody (2D3) [H00023636-M02] - NUP62 monoclonal antibody (M02), clone 2D3 Analysis of NUP62 expression in Hela S3 NE.
Western Blot: NUP62 Antibody (2D3) [H00023636-M02]

Western Blot: NUP62 Antibody (2D3) [H00023636-M02]

Western Blot: Nucleoporin p62 Antibody (2D3) [H00023636-M02] - Analysis of NUP62 expression in transfected 293T cell line by NUP62 monoclonal antibody (M02), clone 2D3.Lane 1: NUP62 transfected lysate (Predicted MW: 53.3 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Nucleoporin p62 Antibody (2D3) [H00023636-M02] - Detection limit for recombinant GST tagged NUP62 is approximately 1ng/ml as a capture antibody.

Applications for NUP62 Antibody (2D3) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NUP62

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform.

Alternate Names

DKFZp547L134,62 kDa nucleoporin, FLJ20822, FLJ43869, IBSNp62, MGC841, nuclear pore glycoprotein p62, nucleoporin 62kD, nucleoporin 62kDa, SNDINucleoporin Nup62

Entrez Gene IDs

23636 (Human)

Gene Symbol

NUP62

OMIM

605815 (Human)

UniProt

NP_714941 (Human)

Additional NUP62 Products

Product Documents for NUP62 Antibody (2D3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for NUP62 Antibody (2D3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NUP62 Antibody (2D3) - Azide and BSA Free

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Protocols

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FAQs

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