O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25586

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse, Rat, Drosophila

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1641 expressed in HEK293

Format

Azide and BSA Free
View all O-GlcNAc Transferase/OGT Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human O-GlcNAc Transferase p110 subunit. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images

O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] -

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (NBP3-25586) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] -

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] - O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (NBP3-25586) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] -

Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] - O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (NBP3-25586) diluted at 1:500. Blue: Fluoroshield with DAPI.
O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] -

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] - O-GlcNAc transferase antibody [HL1641] detects O-GlcNAc transferase protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody [HL1641] (NBP3-25586) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] -

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (NBP3-25586) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] -

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free [NBP3-25586] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody [HL1641] (NBP3-25586) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
O-GlcNAc Transferase p110 subunit Antibody (HL1641) - Azide and BSA Free

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (HL1641) [NBP3-25586] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc Transferase p110 subunit antibody [HL1641] (NBP3-25586) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: O-GlcNAc Transferase/OGT

Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.

Long Name

O-Linked N-Acetylglucosamine (GlcNAc) Transferase

Alternate Names

HRNT1, OGlcNAc Transferase, OGT

Gene Symbol

OGT

Additional O-GlcNAc Transferase/OGT Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

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Protocols

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