Occludin Antibody (CL1555)

Name: Occludin Antibody (CL1555)
Brand: Novus Biologicals
SKU: NBP3-52053
Price: 579 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-52053

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG_2A Clone # CL1555

View all Occludin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q16625, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Occludin Antibody (CL1555)

Immunocytochemistry/ Immunofluorescence: Occludin Antibody (CL1555) [NBP3-52053]

Staining of CACO-2 cells showing specific staining in cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry: Occludin Antibody (CL1555) [NBP3-52053]

Staining of human colon shows strong membranous positivity in glandular cells.

Western Blot: Occludin Antibody (CL1555) [NBP3-52053]

Lane 1: Marker [kDa]
Lane 2: Human cell line CACO-2
Lane 3: Human cell line U-87

Immunohistochemistry: Occludin Antibody (CL1555) [NBP3-52053]

Analysis in human stomach and skeletal muscle tissues. Corresponding Occludin RNA-seq data are presented for the same tissues.

Immunohistochemistry: Occludin Antibody (CL1555) [NBP3-52053]

Staining of human liver shows membranous immunoreactivity in hepatocytes.

Immunohistochemistry: Occludin Antibody (CL1555) [NBP3-52053]

Staining of human fallopian tube shows strong positivity in apical membrane in glandular cells.

Immunohistochemistry: Occludin Antibody (CL1555) [NBP3-52053]

Staining of human prostate cancer shows strong apical membrane positivity in tumor cells.

Immunohistochemistry: Occludin Antibody (CL1555) [NBP3-52053]

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry: Occludin Antibody (CL1555) [NBP3-52053]

Staining of human stomach shows strong positivity in apical membrane in glandular cells.

Immunohistochemistry: Occludin Antibody (CL1555) [NBP3-52053]

Staining of human kidney shows moderate to strong positivity in apical membrane in cells in tubules.

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Applications for Occludin Antibody (CL1555)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Occludin

The Occludin gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction.

Alternate Names

BLCPMG, OCLN

Gene Symbol

OCLN

Additional Occludin Products

Product Documents for Occludin Antibody (CL1555)

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Certificate of Analysis

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Product Specific Notices for Occludin Antibody (CL1555)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.