Occludin Antibody (CL1608) - BSA Free

Name: Occludin Antibody (CL1608) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-52973
Price: 329 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-52973

Submit a review

Citations (1)

Product Documents

View Available Conjugates View Available Formulations

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # CL1608

Format

BSA Free

View all Occludin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q16625, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (83%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Occludin Antibody (CL1608) - BSA Free

Western Blot: Occludin Antibody (CL1608) [NBP2-52973]

Western Blot: Occludin Antibody (CL1608) [NBP2-52973] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: OCLN Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Immunocytochemistry/ Immunofluorescence: Occludin Antibody (CL1608) [NBP2-52973]

Immunocytochemistry/Immunofluorescence: Occludin Antibody (CL1608) [NBP2-52973] - Staining of CACO-2 cells using the Anti-OCLN monoclonal antibody, showing specific staining in cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973] - Staining of human tonsil shows membranous immunoreactivity in squamous epithelium cells.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973] - Staining of human breast cancer shows membranous immunoreactivity in tumor cells.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973] - Staining of human colon shows strong membranous immunoreactivity in glandular cells.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973] - Staining of human kidney shows membranous positivity in renal tubules.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973] - Staining of human liver shows membranous immunoreactivity in hepatocytes.

Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973]

Immunohistochemistry-Paraffin: Occludin Antibody (CL1608) [NBP2-52973] - Staining of human prostate cancer shows membranous positivity in tumor cells.

View 5 more images

Applications for Occludin Antibody (CL1608) - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml

Application Notes

IHC-P,Retrieval method: HIER pH6, ICC/IF,Fixation/Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Occludin

The Occludin gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction.

Alternate Names

BLCPMG, OCLN

Gene Symbol

OCLN

Additional Occludin Products

Product Documents for Occludin Antibody (CL1608) - BSA Free

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Occludin Antibody (CL1608) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.