OCRL Antibody (4A6) - Azide and BSA Free

Novus Biologicals | Catalog # H00004952-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4A6

Format

Azide and BSA Free
View all OCRL Primary Antibodies »

Product Specifications

Immunogen

OCRL (NP_000267, 146 a.a. ~ 239 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SGLLGFEDNFSSMNLDKKINSQNQPTGIHREPPPPPFSVNKMLPREKEASNKEQPKVTNTMRKLFVPNTQSGQREGLIKHILAKREKEYVNIQT

Specificity

OCRL - oculocerebrorenal syndrome of Lowe

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for OCRL Antibody (4A6) - Azide and BSA Free

Western Blot: OCRL Antibody (4A6) [H00004952-M02]

Western Blot: OCRL Antibody (4A6) [H00004952-M02]

Western Blot: OCRL Antibody (4A6) [H00004952-M02] - Analysis of OCRL expression in transfected 293T cell line by OCRL monoclonal antibody (M02), clone 4A6.Lane 1: OCRL transfected lysate(103.2 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: OCRL Antibody (4A6) [H00004952-M02]

Immunohistochemistry-Paraffin: OCRL Antibody (4A6) [H00004952-M02]

Immunohistochemistry-Paraffin: OCRL Antibody (4A6) [H00004952-M02] - Analysis of monoclonal antibody to OCRL on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
Western Blot: OCRL Antibody (4A6) [H00004952-M02]

Western Blot: OCRL Antibody (4A6) [H00004952-M02]

Western Blot: OCRL Antibody (4A6) [H00004952-M02] - Analysis of OCRL over-expressed 293 cell line, cotransfected with OCRL Validated Chimera RNAi ( Cat # H00004952-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with OCRL monoclonal antibody (M02), clone 4A6 (Cat # H00004952-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: OCRL Antibody (4A6) [H00004952-M02]

Western Blot: OCRL Antibody (4A6) [H00004952-M02]

Western Blot: OCRL Antibody (4A6) [H00004952-M02] - OCRL monoclonal antibody (M02), clone 4A6 Analysis of OCRL expression in Hela S3 NE.
Sandwich ELISA: OCRL Antibody (4A6) [H00004952-M02] - Detection limit for recombinant GST tagged OCRL is approximately 0.1ng/ml as a capture antibody.

Applications for OCRL Antibody (4A6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: OCRL

This gene encodes a phosphatase enzyme that is involved in actin polymerization and is found in the trans-Golgi network. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease.

Alternate Names

EC 3.1.3.36, INPP5F, LOCR, Lowe oculocerebrorenal syndrome protein, NPHL2, OCRL1inositol polyphosphate 5-phosphatase OCRL-1, oculocerebrorenal syndrome of Lowe, phosphatidylinositol polyphosphate 5-phosphatase

Entrez Gene IDs

4952 (Human)

Gene Symbol

OCRL

OMIM

300009 (Human)

UniProt

NP_000267 (Human)

Additional OCRL Products

Product Documents for OCRL Antibody (4A6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for OCRL Antibody (4A6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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