OCT4 Antibody (486) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-15052

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rabbit

Predicted:

Chimpanzee (100%), Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 486

Format

Azide and BSA Free
View all OCT4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human OCT4. The exact sequence is proprietary.

Reactivity Notes

Feline (89%), Porcine (88%),Bovine (85%). Rabbit reactivity reported from a verified customer review.

Marker

Embryonic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OCT4 Antibody (486) - Azide and BSA Free

Western Blot: OCT4 Antibody (486) [NBP2-15052]

Western Blot: OCT4 Antibody (486) [NBP2-15052]

Western Blot: OCT4 Antibody (486) [NBP2-15052] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052]

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052]

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Paraffin-embedded human cervical carcinoma.Oct4 stained by Oct4 antibody [GT486] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Flow Cytometry: OCT4 Antibody (486) [NBP2-15052]

Flow Cytometry: OCT4 Antibody (486) [NBP2-15052]

Flow Cytometry: OCT4 Antibody (486) [NBP2-15052] - Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody dilution: 1:50
Western Blot: OCT4 Antibody (486) [NBP2-15052]

Western Blot: OCT4 Antibody (486) [NBP2-15052]

Western Blot: OCT4 Antibody (486) [NBP2-15052] - Sample (10 ug of whole cell lysate) A: Non-transfected HeLa lysates B: HeLa transfected recombinant OCT3/4 protein 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (486) [NBP2-15052]

Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (486) [NBP2-15052]

Immunocytochemistry/Immunofluorescence: OCT4 Antibody (486) [NBP2-15052] - Human embryonic stem cell were fixed in 4% Paraformaldehyde for 15 min. Green: Oct4 protein stained by Oct4 antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052]

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052]

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - BT483 xenograft, using OCT3/4 antibody at 1:200 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052]

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052]

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Imaging of Rabbit stem cels in Bone marrow of tibia. This image was submitted via customer Review.
Immunoprecipitation: OCT4 Antibody (486) [NBP2-15052]

Immunoprecipitation: OCT4 Antibody (486) [NBP2-15052]

Immunoprecipitation: OCT4 Antibody (486) [NBP2-15052] - Sample: cell lysate/extract of OCT4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell, B. Control with 2 ug of preimmune mouse IgG, C. Immunoprecipitation of OCT4 by 2 ug of OCT4 antibody 12% SDS-PAGE gel.
OCT4 Antibody (486)

Western Blot: OCT4 Antibody (486) [NBP2-15052] -

Western Blot: OCT4 Antibody (486) [NBP2-15052] - Mouse embryonic stem cell extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (NBP2-15052) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
OCT4 Antibody (486)

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] -

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Oct4 stained by Oct4 antibody [GT486] (NBP2-15052) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
OCT4 Antibody (486)

Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (486) [NBP2-15052] -

Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (486) [NBP2-15052] - Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Oct4 stained by Oct4 antibody [GT486] (NBP2-15052) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.
OCT4 Antibody (486)

Western Blot: OCT4 Antibody (486) [NBP2-15052] -

Western Blot: OCT4 Antibody (486) [NBP2-15052] - Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] diluted at 1:120000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
OCT4 Antibody (486) - Azide and BSA Free

Western Blot: OCT4 Antibody (486) [NBP2-15052] -

Oct4 antibody [GT486] detects Oct4 protein by western blot analysis.
A. 30 ug human ESC whole cell lysate/extract
10% SDS-PAGE
(NBP2-15052) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for OCT4 Antibody (486) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:1000-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP2-15052 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: OCT4

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

Alternate Names

class 5, transcription factor 1, MGC22487, Oct-3, OCT3Oct4, Oct-4, Octamer-binding protein 3, otc-4, OTF3POU domain class 5, transcription factor 1, OTF4, POU class 5 homeobox 1, POU-type homeodomain-containing DNA-binding protein

Gene Symbol

POU5F1

Additional OCT4 Products

Product Documents for OCT4 Antibody (486) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for OCT4 Antibody (486) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for OCT4 Antibody (486) - Azide and BSA Free

Customer Reviews for OCT4 Antibody (486) - Azide and BSA Free (1)

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Customer Images

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  • OCT4 Antibody (486)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: tibia tissue
    Species: Rabbit
    Verified Customer | Posted 03/16/2017
    stem cell at Bone marrow.
    OCT4 Antibody (486) - Azide and BSA Free NBP2-15052

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Protocols

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