OGDH Antibody

Novus Biologicals | Catalog # NBP2-19622

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (97%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all OGDH Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human OGDH. The exact sequence is proprietary.

Reactivity Notes

Chicken (87%), Xenopus laevis (84%),

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OGDH Antibody

Immunocytochemistry/ Immunofluorescence: OGDH Antibody [NBP2-19622]

Immunocytochemistry/ Immunofluorescence: OGDH Antibody [NBP2-19622]

Immunocytochemistry/Immunofluorescence: OGDH Antibody [NBP2-19622] - OGDH antibody detects OGDH protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: OGDH stained by OGDH antibody diluted at 1:200. Blue: Fluoroshield with DAPI. Scale bar= 10 um.
Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622]

Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622]

Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622] - Mouse heart. OGDH stained by OGDH antibody [C2C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: OGDH Antibody [NBP2-19622]

Western Blot: OGDH Antibody [NBP2-19622]

Western Blot: OGDH Antibody [NBP2-19622] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:5000.
Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622]

Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622]

Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622] - Cal27 xenograft, using OGDH antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622]

Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622]

Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622] - Mouse muscle. OGDH antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
OGDH Antibody

Western Blot: OGDH Antibody [NBP2-19622] -

Western Blot: OGDH Antibody [NBP2-19622] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OGDH antibody [C2C3], C-term (NBP2-19622) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
OGDH Antibody

Western Blot: OGDH Antibody [NBP2-19622] -

OGDH antibody detects OGDH protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and blotted with OGDH antibody (NBP2-19622) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
OGDH Antibody

Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622] -

OGDH antibody [C2C3], C-term detects OGDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
OGDH stained by OGDH antibody [C2C3], C-term (NBP2-19622) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for OGDH Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: OGDH

This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitocondrial matrix and uses thiamine pyrophosphate as a cofactor. A congential deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. [provided by RefSeq]

Long Name

Oxoglutarate Dehydrogenase

Alternate Names

E1k, KGD1, OGDC, OGDC-E1, OGDH2

Gene Symbol

OGDH

UniProt

Q02218 (Human)

Additional OGDH Products

Product Documents for OGDH Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for OGDH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Redox Enzymes

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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