Okadaic acid

Tocris Bioscience | Catalog # 1136

Protein phosphatase 1 and 2A inhibitor
Tocris Bioscience

Key Product Details

Description

Protein phosphatase 1 and 2A inhibitor
View all Protein Ser/Thr Phosphatase Inhibitors »

Product Description

Okadaic acid is a potent inhibitor of protein phosphatase 1 (IC50 = 3 nM) and protein phosphatase 2A (IC50 = 0.2-1 nM). Displays > 100,000,000-fold selectivity over PP2B and PP2C. Tumor promoter. Shown to activate atypical protein kinase C in adipocytes.

Okadaic acid ammonium salt (Cat. No. 9012) also available.

Product Specifications for Okadaic acid

Molecular Weight

805.01

Formula

C44H68O13

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

9,10-Deepithio-9,10-didehydroacanthifolicin

CAS Number

78111-17-8

PubChem ID

90479758

InChI Key

QNDVLZJODHBUFM-WFXQOWMNSA-N

SMILES

[H][C@]1(CC(C)=C[C@@]2(O[C@H](C[C@@](C)(O)C(O)=O)CC[C@H]2O)O1)[C@H](C)\C=C\[C@H]1CC[C@@]2(CC[C@@]3([H])O[C@]([H])([C@@H](O)C[C@H](C)[C@@]4([H])O[C@@]5(CCCCO5)CC[C@H]4C)C(=C)[C@@H](O)[C@]3([H])O2)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 40 50

Preparing Stock Solutions for Okadaic acid

The following data is based on the product molecular weight 805.01.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 2.48 mL 12.42 mL 24.84 mL
2.5 mM 0.50 mL 2.48 mL 4.97 mL
5 mM 0.25 mL 1.24 mL 2.48 mL
25 mM 0.05 mL 0.25 mL 0.50 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 132 publications citing the usage of this product.

Product Documents for Okadaic acid

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Okadaic acid

For research use only

Related Research Areas

Epigenetics
Phosphatases and Regulators

Customer Reviews for Okadaic acid (2)

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  • Works as intended.
    Name: Anonymous
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: Sprague-Dawley rats primary neuronal
    Verified Customer | Posted 04/04/2020
    Okadaic acid for PP2A at 20 nM
    Okadaic acid 1136
  • Okadaic acid increases phosphorylation of CaMKII in HL-1 cells
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Cardiomyocytes
    Verified Customer | Posted 05/15/2019
    HL-1 cardiomyocytes were treated with 100 nM fostriecin, 100 nM Okadaic acid or 50 nM 8-bromo-cAMP for 30 min followed by Western blot analysis for pCaMKII expression. Okadaic acid significantly increased phosphorylation of CaMKII; indicating a major role of PP1 in CaMKII dephosphorylation.
    Okadaic acid 1136

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