Olig1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-28666

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Olig1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant mouse Olig1. Accession # Q9JKN5

Specificity

Specific for endogenous levels of the ~27 kDa Olig1 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for Olig1 Antibody - Azide and BSA Free

Western Blot: Olig1 AntibodyAzide and BSA Free [NBP1-28666]

Western Blot: Olig1 AntibodyAzide and BSA Free [NBP1-28666]

Western Blot: OLIG1 Antibody [NBP1-28666] - Neonatal rat brain lysate showing specific immunolabeling of the ~ 27k Olig1 protein.
Immunocytochemistry/ Immunofluorescence: Olig1 Antibody - Azide and BSA Free [NBP1-28666]

Immunocytochemistry/ Immunofluorescence: Olig1 Antibody - Azide and BSA Free [NBP1-28666]

Immunocytochemistry/Immunofluorescence: Olig1 Antibody [NBP1-28666] - Immunostaining of primary rat oligodendrocytes using anti-Olig1.

Applications for Olig1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Immunoprecipitation

1:50

Western Blot

1:3000
Application Notes
Detects ~27 kDa Olig1 protein in Western blots.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

200mM Tris (pH 8.0) and 0.1M Glycine

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Olig1

Olig genes have been identified as the earliest known markers of oligodendrocyte lineage determination to date (Zhou et al., 2000). Olig1 is a transcription factor which promotes formation and maturation of oligodendrocytes, especially within the brain. It is expressed in the ventral spinal cord as early as 9.5 dpc and by 15.5 dpc, olig1 is dispersed throughout the gray matter. In the postnatal brain, it is present preferentially in the white matter, such as corpus callosum and cerebellar medulla. Olig1 has been demonstrated as necessary in the repair of brain lesions in patients with multiple sclerosis (Arnett et al. 2004).

Long Name

Oligodendrocyte Lineage Gene 1

Alternate Names

BHLHB6

Entrez Gene IDs

50914 (Mouse); 60394 (Rat)

Gene Symbol

OLIG1

UniProt

Q9JKN5 (Mouse)

Additional Olig1 Products

Product Documents for Olig1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Olig1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Olig1 Antibody - Azide and BSA Free

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Protocols

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FAQs

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