Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 064 expressed in HEK293

Format

Azide and BSA Free
View all Olig2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, synthetic peptide corresponding to the N-terminus of the Human Olig2.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Olig2 Antibody (064) - Azide and BSA Free

Western Blot: Olig2 Antibody (064) [NBP2-89201]

Western Blot: Olig2 Antibody (064) [NBP2-89201]

Western Blot: Olig2 Antibody (064) [NBP2-89201] - A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:37 kDa
Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201]

Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201]

Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201] - Staining of human olig-2 in human brain with rabbit monoclonal antibody (1:200).
Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201]

Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201]

Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201] - Staining of human olig-2 in human glioma with rabbit monoclonal antibody (1:200).
Olig2 Antibody (064)

Flow Cytometry: Rabbit Monoclonal Olig2 Antibody (064) [NBP2-89201] -

Flow Cytometry: Rabbit Monoclonal Olig2 Antibody (064) [NBP2-89201] - Mouse brain cells fixed and stained for Olig2 protein: 1:100. AF405 conjugated version of antibody used (Catalog # NBP2-89201AF405). Image from a verified customer review.

Olig2 Antibody (064)

Flow Cytometry: Rabbit Monoclonal Olig2 Antibody (064) [NBP2-89201] -

Flow Cytometry: Rabbit Monoclonal Olig2 Antibody (064) [NBP2-89201] - Rat brain cells fixed and stained with Olig2. AF405 conjugated version of antibody used (Catalog # NBP2-89201AF405). Image from a verified customer review.

Applications for Olig2 Antibody (064) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Validated for Flow Cytometry from a verified customer review.

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:500-1:2000

Reviewed Applications

Read 1 review rated 5 using NBP2-89201 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Olig2

Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. OIlig2 is expressed in the ventral spinal cord as early as 9.5 dpc and is scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. (Arnett et al., 2004) From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007).

Long Name

Oligodendrocyte Lineage Transcription Factor 2

Alternate Names

BHLHB1, PRKCBP2, RACK17

Gene Symbol

OLIG2

Additional Olig2 Products

Product Documents for Olig2 Antibody (064) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Olig2 Antibody (064) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Olig2 Antibody (064) - Azide and BSA Free

Customer Reviews for Olig2 Antibody (064) - Azide and BSA Free (1)

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Customer Images

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  • Olig2 Antibody (064)
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Adult mouse brain
    Species: Mouse
    Verified Customer | Posted 04/02/2024
    mouse brain cells fixed and stained for olig2 protein : 1:100 NBP2-89201AF405 (Alexa fluor 405)
    Olig2 Antibody (064) - Azide and BSA Free NBP2-89201
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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FAQs

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