Olig2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88634

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Olig2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SPEPDDLFLPARSKGSSGSAFTGGTVSSSTPSDCPPELSAELRGAMGSAGAHPGDKLGGSGFKSSSSSTSSSTSSAAASSTKKDKKQMTEPELQQLRLKINSRERKRMHDLNI

Marker

Oligodendrocyte Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Olig2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Olig2 Antibody [NBP1-88634]

Immunohistochemistry-Paraffin: Olig2 Antibody [NBP1-88634]

Immunohistochemistry-Paraffin: Olig2 Antibody [NBP1-88634] - Staining in human cerebral cortex and liver tissues. Corresponding OLIG2 RNA-seq data are presented for the same tissues.
Western Blot: Olig2 Antibody [NBP1-88634]

Western Blot: Olig2 Antibody [NBP1-88634]

Western Blot: Olig2 Antibody [NBP1-88634] - Analysis in human cerebral cortex tissue.
Immunohistochemistry-Paraffin: Olig2 Antibody [NBP1-88634]

Immunohistochemistry-Paraffin: Olig2 Antibody [NBP1-88634]

Immunohistochemistry-Paraffin: Olig2 Antibody [NBP1-88634] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Olig2 Antibody [NBP1-88634]

Immunohistochemistry-Paraffin: Olig2 Antibody [NBP1-88634]

Immunohistochemistry-Paraffin: Olig2 Antibody [NBP1-88634] - Staining of human cerebral cortex shows moderate nuclear positivity in oligodendrocytes.
Immunohistochemistry-Paraffin: Olig2 Antibody [NBP1-88634]

Immunohistochemistry-Paraffin: Olig2 Antibody [NBP1-88634]

Immunohistochemistry-Paraffin: Olig2 Antibody [NBP1-88634] - Staining of human glioma shows moderate nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: Olig2 Antibody [NBP1-88634]

Immunohistochemistry-Paraffin: Olig2 Antibody [NBP1-88634]

Immunohistochemistry-Paraffin: Olig2 Antibody [NBP1-88634] - Staining of human liver shows no positivity as expected.
Olig2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: Olig2 Antibody - BSA Free [NBP1-88634]

Chromatin Immunoprecipitation-exo-Seq: Olig2 Antibody - BSA Free [NBP1-88634]

ChIP-Exo-Seq composite graph for Anti-OLIG2 (NBP1-88634) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for Olig2 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Olig2

Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. OIlig2 is expressed in the ventral spinal cord as early as 9.5 dpc and is scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. (Arnett et al., 2004) From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007).

Long Name

Oligodendrocyte Lineage Transcription Factor 2

Alternate Names

BHLHB1, PRKCBP2, RACK17

Entrez Gene IDs

10215 (Human)

Gene Symbol

OLIG2

UniProt

Q13516

Additional Olig2 Products

Product Documents for Olig2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Olig2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Olig2 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Olig2 Antibody - BSA Free and earn rewards!

Have you used Olig2 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies