Oligodendrocyte Marker O4 APC-conjugated Antibody

  
  • Species Reactivity
    Human, Mouse, Rat, Chicken
  • Specificity
    Detects human, mouse, rat, and chicken Oligodendrocyte Marker O4.
  • Source
    Monoclonal Mouse IgM Clone # O4
  • Purification
    IgM-specific Affinity-purified from hybridoma culture supernatant
  • Immunogen
    Bovine brain corpus callosum white matter
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Oligodendrocyte Marker O4 in Differentiated Rat Cortical Stem Cells by Flow Cytometry. Differentiated Rat Cortical Stem Cells (Catalog # NSC001) were stained with Mouse Anti-Human/Mouse/
Rat/Chicken Oligodendrocyte Marker O4 APC‑conjugated Monoclonal Antibody (Catalog # FAB1326A, filled histogram) or isotype control antibody (Catalog # IC015A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Oligodendrocyte Marker O4

Oligodendrocytes are myelinating cells in the central nervous system (CNS) and form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O4 is an antigen on the surface of oligodendrocyte progenitors (1, 2). It has been commonly used as the earliest recognized marker specific for the oligodendroglial lineage (3-8).

  • References:
    1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
    2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
    3. Bansal, R. and Pfeiffer, S.E. (1989) Proc. Natl. Acad. Sci. USA 86:6181.
    4. Gard, A. et al. (1995) Dev. Biol. 167:596.
    5. Reynolds, R. and Hardy, R. (1997) J. Neurosci. Res. 47:455.
    6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
    7. Pang, Y. et al. (2000) J. Neurosci. Res. 62:510.
    8. Cai, Z. et al. (2001) Brain Res. 898:126.
  • Alternate Names:
    Oligodendrocyte Marker O4
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Mouse IgM APC-conjugated Control

Ctrl IC015A
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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