Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgM Clone # O4
View all Oligodendrocyte Marker O4 Primary Antibodies »

Product Specifications

Immunogen

Bovine brain corpus callosum white matter

Specificity

Detects human, mouse, rat, and chicken Oligodendrocyte Marker O4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for  Oligodendrocyte Marker O4 APC-conjugated Antibody

Detection of Oligodendrocyte Marker O4 antibody in Differentiated Rat Cortical Stem Cells antibody by Flow Cytometry.

Detection of Oligodendrocyte Marker O4 in Differentiated Rat Cortical Stem Cells by Flow Cytometry.

Differentiated Rat Cortical Stem Cells (Catalog # NSC001) were stained with Mouse Anti-Human/Mouse/ Rat/Chicken Oligodendrocyte Marker O4 APC-conjugated Monoclonal Antibody (Catalog # FAB1326A, filled histogram) or isotype control antibody (Catalog # IC015A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for  Oligodendrocyte Marker O4 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Differentiated Rat Cortical Stem Cells (Catalog # NSC001)

Reviewed Applications

Read 2 reviews rated 4.5 using FAB1326A in the following applications:

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oligodendrocyte Marker O4

Oligodendrocytes are myelinating cells in the central nervous system (CNS) and form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O4 is an antigen on the surface of oligodendrocyte progenitors (1, 2). It has been commonly used as the earliest recognized marker specific for the oligodendroglial lineage (3-8).

References

  1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
  2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
  3. Bansal, R. and Pfeiffer, S.E. (1989) Proc. Natl. Acad. Sci. USA 86:6181.
  4. Gard, A. et al. (1995) Dev. Biol. 167:596.
  5. Reynolds, R. and Hardy, R. (1997) J. Neurosci. Res. 47:455.
  6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
  7. Pang, Y. et al. (2000) J. Neurosci. Res. 62:510.
  8. Cai, Z. et al. (2001) Brain Res. 898:126.

Additional Oligodendrocyte Marker O4 Products

Product Documents for  Oligodendrocyte Marker O4 APC-conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for  Oligodendrocyte Marker O4 APC-conjugated Antibody

For research use only

Related Research Areas

Glial Lineage Markers

Citations for  Oligodendrocyte Marker O4 APC-conjugated Antibody

Customer Reviews for  Oligodendrocyte Marker O4 APC-conjugated Antibody (2)

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2 Customer Ratings
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  • Oligodendrocyte Marker O4 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 08/26/2025
     Oligodendrocyte Marker O4 APC-conjugated Antibody FAB1326A
  • Oligodendrocyte Marker O4 APC-conjugated Antibody
    Name: Nuria Álvarez-Sánchez
    Application: Flow Cytometry
    Sample Tested: brain and spinal cord
    Species: Mouse
    Verified Customer | Posted 02/07/2019
    CNS cells of EAE mice stained for O4. Viable non-stained cells (blue) and stained cells (red).
    Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and cells from central nervous system (brain+spinal cord) were isolated when the animals were at the peak of the disease. Cells were stained for MOG, O4, GalC and AN2, plus for viability to exclude dead cells. MOG antibody was biotinylated, so cells were subsequently incubated with streptavidin-BV786 to stain MOG+ cells. Viable cells were gated and O4 staining is showed in non-stained cells (blue histogram) and in stained cells (red histogram).
     Oligodendrocyte Marker O4 APC-conjugated Antibody FAB1326A

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