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OLLAS Epitope Tag Antibody (L2) - BSA Free

Novus Biologicals | Catalog # NBP1-06713

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Epitope Tag

Cited:

Human, Mouse, Hamster - Cricetulus (Chinese Hamster), Insect - Drosophila, Nematode - Caenorhabditis elegans, Parasite

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Whole-Mount, Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation Sequencing

Cited:

Immunohistochemistry, Immunohistochemistry Whole-Mount, Western Blot, Immunoblotting, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chip Cytometry, IF/IHC, Mass Spectrometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 kappa Clone # L2

Format

BSA Free
View all OLLAS Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

OLLAS (SGFANELGPRLMGK)-tagged extracellular domain of mouse Langerin

Clonality

Monoclonal

Host

Rat

Isotype

IgG1 kappa

Scientific Data Images for OLLAS Epitope Tag Antibody (L2) - BSA Free

Western Blot: OLLAS Epitope Tag Antibody (L2)BSA Free [NBP1-06713]

Western Blot: OLLAS Epitope Tag Antibody (L2)BSA Free [NBP1-06713]

Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - C. elegans whole nematode. OLLAS::PRMT-1 Western Blot using NBP1-06713SS (1:1000). Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713]

Immunocytochemistry/ Immunofluorescence: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713]

Immunocytochemistry/Immunofluorescence: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Live cell imaging of GFP-tagged MES-2/EZH2 (the catalytic subunit of C. elegans PRC2), and immunofluorescence of H3K27me3 and H3.3/oncohistone (tagged with OLLAS Epitope Tag) in pachytene nuclei of H3-like K27M oncohistone (H3-like mut) worms. Scale bars represent 5 um. Chromosome X was identified by depletion of H3.3 and H3K4me3 staining shown in Supplementary Fig. 3, and is marked with an asterisk. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license Image using the Biotin format of this antibody.
Immunohistochemistry: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713]

Immunohistochemistry: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713]

OLLAS-Epitope-Tag-Antibody-L2-BSA-Free-Immunohistochemistry-NBP1-06713-img0010.jpg
Western Blot: OLLAS Epitope Tag Antibody (L2)BSA Free [NBP1-06713]

Western Blot: OLLAS Epitope Tag Antibody (L2)BSA Free [NBP1-06713]

Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies to OLLAS (NBP1-06713), DYKDDDDK (NBP1-06712) and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG (TM) and ANTI-FLAG (TM) are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
Western Blot: OLLAS Epitope Tag Antibody (L2)BSA Free [NBP1-06713]

Western Blot: OLLAS Epitope Tag Antibody (L2)BSA Free [NBP1-06713]

Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - OLLAS tagged SFO-p41 protein.
Immunohistochemistry Whole-Mount: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713]

Immunohistochemistry Whole-Mount: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713]

Immunohistochemistry Whole-Mount: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Drosophila adult midgut expressing an OLLAS-tagged protein stained with OLLAS Epitope Tag Antibody (magenta). Nuclei are shown in green. IHC image submitted by a verified customer review.
Immunohistochemistry-Frozen: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713]

Immunohistochemistry-Frozen: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713]

Immunohistochemistry-Frozen: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Vector Map of pCMV-SD OLLAS
Immunohistochemistry-Paraffin: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713]

Immunohistochemistry-Paraffin: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713]

OLLAS-Epitope-Tag-Antibody-L2-Immunohistochemistry-Paraffin-NBP1-06713-img0008.jpg
Immunohistochemistry-Paraffin: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713]

Immunohistochemistry-Paraffin: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713]

OLLAS-Epitope-Tag-Antibody-L2-Immunohistochemistry-Paraffin-NBP1-06713-img0007.jpg
OLLAS Epitope Tag Antibody (L2) - BSA Free

Western Blot: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713] -

Western Blot: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713] - Utilization of pMVP for the creation of unique PDX1 vectors. pENTR plasmids containing cDNA for human PDX1 with or without a stop codon (i.e. open) were recombined with pMVP components to generate an assortment of PDX1-expressing (A) adenovirus & (C) expression plasmid vectors. (B) Immunoblot blot analysis of INS1 832/13 cell lysates harvested 48h after treatment with crude adenovirus lysates. For the epitope tagged conditions, note the appearance of the endogenous (lower) & overexpressed (upper) PDX1 bands (top blot). For eGFP blot, the use of P2A adds 23 amino acids to the N-terminal protein (i.e. eGFP). Immunoblot analysis of HEK293 cell lysates 24 h after transfection of expression vectors encoding PDX1 with C-terminal (D) epitope tags, (E) eGFP reporter or fusion, (G) & mCherry reporter or fusion. (F, H) Fluorescence microscopy of the eGFP & mCherry containing conditions analyzed in panels E & G. Visible bands resulting from ‘Uncleaved’ P2A products (red circle •) & degradation products (*) are labeled. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30590691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
OLLAS Epitope Tag Antibody (L2) - BSA Free

Immunocytochemistry/ Immunofluorescence: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713] -

MES proteins are expressed in nos-1nos-2 embryonic PGCs.Transcriptome comparison between PGCs isolated from wild-type and mes-2(RNAi) L1 larvae. (A) Volcano plot showing log2 fold change of gene expression between mes-2(RNAi) and wild-type L1 PGCs. The numbers of genes whose expression were up or downregulated in mes-2(RNAi) compared to wild-type L1 PGCs are indicated. Dashed lines mark the significance cutoff of q = 0.05 above which genes were counted as misexpressed. (B) Bar graph showing chromosomal distribution of mes-2(RNAi) upregulated genes. Asterisks indicate significantly more genes than expected (hypergeometric test, p-value<0.001 [**]). (C) Top: Photomicrograph of live embryo expressing GFP tagged MES-2 in wild- type and nos-1(gv5)nos-2(ax3103) embryos. Middle: Photomicrograph of fixed wild-type and nos-1(gv5)nos-2(ax3103) embryos expressing OLLAS tagged MES-3. Bottom: Photomicrograph of fixed wild-type and nos-1(gv5)nos-2(RNAi) embryos stained with alpha -MES-4 antibody and K76 alpha -PGL-1 antibody. Images of 2-fold+ stage embryos were taken. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29111977), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
OLLAS Epitope Tag Antibody (L2) - BSA Free

Immunocytochemistry/ Immunofluorescence: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713] -

Comparison of localization of the CD19::Nrx, nSyb::CD19 and CD19::sdc ligands into presynaptic sites in the ORNs targeting DA1.Different intracellular and transmembrane domains (from Nrx, nSyb, and sdc) were fused to CD19 and expressed into ORNs targeting the DA1 glomerulus using the CD19::Nrx (top panels), nSyb::CD19 (middle panels) and CD19::Sdc (bottom panels), with the R17H02 driver (in green). The brain samples were co-immunostained with antibodies against the OLLAS tag (present in the ligand) and against the pre-synaptic protein, BRP (in magenta). The ligand proteins are co-localized with or adjacent to BRP (arrows), demonstrating that all ligands are enriched at the presynaptic terminals of the ORNs, but CD19::Nrx was also expressed at strong levels in the axon shaft outside of the glomerulus (arrowheads in top panels). Scale bar = 20 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29231171), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for OLLAS Epitope Tag Antibody (L2) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation Sequencing

reported in scientific literature (PMID 31175278)

Immunoblotting

reported in scientific literature (PMID 27600537)

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:10 - 1:100

Immunohistochemistry-Frozen

1:10 - 1:100

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500 - 1:1000

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-06713 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OLLAS Epitope Tag

OLLAS (Escherichia coli OmpF Linker and mouse Langerin fusion Sequence) epitope tag is a relatively newly discovered tag which is a 14 amino acid sequence residing in the junction between E. coli OmpF protein (OFL/OmpF linker) and mLangerin extracellular domain. OLLAS Epitope Tag antibody (L2), also called MAb L2 or OLLA-2, is a novel rat IgG monoclonal antibody raised against newly identified OLLAS epitope (SGFANELGPRLMGK), with advantages in immunodetection applications. Additionally, this antibody is a rat IgG and can be used for immunostaining of mouse and human samples without direct labelling of fluorochrome or enzyme. Because anti-tag antibodies are mostly generated from mouse and rabbit, the OLLAS Epitope Tag antibody (L2) from rat IgG provides an extra advantage in immunodetection.

Alternate Names

E.coli OmpF Linker and mouse Langerin fusion Sequence, OLLA-2

Additional OLLAS Epitope Tag Products

Product Documents for OLLAS Epitope Tag Antibody (L2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for OLLAS Epitope Tag Antibody (L2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for OLLAS Epitope Tag Antibody (L2) - BSA Free

Customer Reviews for OLLAS Epitope Tag Antibody (L2) - BSA Free (2)

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  • OLLAS Epitope Tag Antibody (L2)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: whole C. elegans nematode
    Species: C. elegans
    Verified Customer | Posted 06/26/2020
    OLLAS::PRMT-1 Western Blot using NBP1-06713SS (1:1000)
    OLLAS Epitope Tag Antibody (L2) - BSA Free NBP1-06713
  • OLLAS Epitope Tag Antibody (L2)
    Name: Masahiko Takemura
    Application: Immunohistochemistry-Whole mount
    Sample Tested: Drosophila adult midgut
    Species: Drosophila
    Verified Customer | Posted 12/12/2016
    A Drosophila adult midgut expressing an OLLAS-tagged protein is stained with the anti-OLLAS antibody (magenta). Nuclei are shown in green.
    OLLAS Epitope Tag Antibody (L2) - BSA Free NBP1-06713

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Protocols

View specific protocols for OLLAS Epitope Tag Antibody (L2) - BSA Free (NBP1-06713):


Western Blot Protocol
1. Perform SDS-PAGE (4-12% MOPS) on samples to be analyzed, loading 5 ug of total protein per lane.
2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
3. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
4. Rinse the blot in TBS for approximately 5 minutes.
5. Block the membrane using 5% NFDM + 1% BSA in TBS + Tween, 1 hour at RT.
6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
7. Dilute the primary antibody (NBP1-06713) in blocking buffer and incubate 1 hour at room temperature.
8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
9. Dilute the appropriate secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided it does not interfere with antibody-antigen binding.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for OLLAS Epitope Tag Antibody (L2) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Can you confirm if you measure the concentration of NBP1-06713 (anti-OLLAS epitope tag)?

    A: Unfortunately, we do not measure the concentration of our OLLAS tag antibody, as the product is provided unpurified.

  • Q: I am interested in tagging proteins from E. coli and am wondering if ompF cross-reactivity is a problem?

    A: The OLLAS tag is not predicted to cross-react with any portion of ompF.

  • Q: Can you confirm if you measure the concentration of NBP1-06713 (anti-OLLAS epitope tag)?

    A: Unfortunately, we do not measure the concentration of our OLLAS tag antibody, as the product is provided unpurified.

  • Q: I am interested in tagging proteins from E. coli and am wondering if ompF cross-reactivity is a problem?

    A: The OLLAS tag is not predicted to cross-react with any portion of ompF.

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