Optineurin Antibody (S01-2C5)

Detection of Optineurin by Immunoprecipitation.

U2OS lysates were prepared, and immunoprecipitation was performed using 2.0 ug of Optineurin antibody (Catalog # NBP3-19900) pre-coupled to Dynabeads protein A. Samples were washed and processed for Western Blot with Optineurin antibody. For Western Blot, Rb x Optineurin was used at 1/1000. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Knockout Validation of Optineurin Antibody in U2OS and Optineurin Knockout U2OS Cells in Immunocytochemistry/Immunofluorescence.

U2OS WT and Optineurin KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Optineurin antibody and with the corresponding Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Antibody dilution used: 1/300. Bars = 10um. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).