Orai1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-77289
![Western Blot: Orai1 AntibodyBSA Free [NBP1-77289]](https://resources.rndsystems.com/images/products/Orai1-Antibody-Western-Blot-NBP1-77289-img0001.jpg "Western Blot: Orai1 AntibodyBSA Free [NBP1-77289]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Rat
Predicted:
Chicken (100%), Rat (93%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1. The immunogen is located within the last 50 amino acids of ORAI1.
Specificity
This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Orai1 Antibody - BSA Free
Western Blot: Orai1 AntibodyBSA Free [NBP1-77289]
Western Blot: Orai1 Antibody - BSA Free [NBP1-77289] - Human ovary tissue lysate with ORAI1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.![Immunohistochemistry-Paraffin: Orai1 Antibody - BSA Free [NBP1-77289]](https://resources.rndsystems.com/images/products/Orai1-Antibody-Immunohistochemistry-NBP1-77289-img0003.jpg "Immunohistochemistry-Paraffin: Orai1 Antibody - BSA Free [NBP1-77289]")
Immunohistochemistry-Paraffin: Orai1 Antibody - BSA Free [NBP1-77289]
Immunohistochemistry-Paraffin: Orai1 Antibody - BSA Free [NBP1-77289] - Analysis of ORAI1 in human ovary tissue with ORAI1 antibody at 2.5 ug/ml.
Immunohistochemistry: Orai1 Antibody - BSA Free [NBP1-77289] -
Immunohistochemistry: Orai1 Antibody - BSA Free [NBP1-77289] - Immunohistochemistry of Orai1 in human spleen tissue with Orai1 antibody at 2.5 u/mL.
Immunocytochemistry/ Immunofluorescence: Orai1 Antibody - BSA Free [NBP1-77289] -
Immunocytochemistry/ Immunofluorescence: Orai1 Antibody - BSA Free [NBP1-77289] - Immunofluorescence of Orai1 in human ovary tissue with Orai1 antibody at 20 ug/mL.Red: Orai1 Antibody
Blue: DAPI staining
Applications for Orai1 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
2.5 ug/ml
Immunohistochemistry-Paraffin
2.5 ug/ml
Western Blot
0.5-2 ug/ml
Application Notes
ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Orai1
Long Name
ORAI Calcium Release-activated Calcium Modulator 1
Alternate Names
CRACM1, ORAT1, TMEM142A
Entrez Gene IDs
84876 (Human)
Gene Symbol
ORAI1
UniProt
Additional Orai1 Products
Product Documents for Orai1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Orai1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Orai1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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