ORP1 Antibody

Novus Biologicals | Catalog # NB100-1331

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ORP1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-YWDRNYFNLPDIY corresponding to C-Terminus according to NP_542164.2.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22241858).

Specificity

This antibody is expected to recognise both reported human isoforms of this protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ORP1 Antibody

Western Blot: ORP1 Antibody [NB100-1331]

Western Blot: ORP1 Antibody [NB100-1331]

Western Blot: ORP1 Antibody [NB100-1331] - (0.5ug/ml) of Human Muscle lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ORP1 Antibody [NB100-1331]

Immunohistochemistry-Paraffin: ORP1 Antibody [NB100-1331]

Immunohistochemistry-Paraffin: ORP1 Antibody [NB100-1331] - (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: ORP1 Antibody [NB100-1331]

Western Blot: ORP1 Antibody [NB100-1331]

Western Blot: ORP1 Antibody [NB100-1331] - Analysis of ORP1 in Human Muscle lysate using NB100-1331 at 0.5 ug/ ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: ORP1 Antibody [NB100-1331]

Immunohistochemistry-Paraffin: ORP1 Antibody [NB100-1331]

Immunohistochemistry-Paraffin: ORP1 Antibody [NB100-1331] - (5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for ORP1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.5-2 ug/ml
Application Notes
Western blot: Approx 48kDa band observed in Human Muscle lysate (predicted MW of 50kDa according to NP_060500).
IHC: Paraffin embedded Human Brain (Cerebellum) and Human Skeletal Muscle.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ORP1

Oxysterols are oxygenated derivatives of cholesterol that have a number of biological effects and play a key role in the maintenance of the body cholesterol balance. ORP1 is a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors that are believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase.

Alternate Names

FLJ10217, ORP1oxysterol-binding protein-related protein 1, ORP-1oxysterol-binding protein-related protein 1 variant 1, OSBP8, OSBPL1, OSBPL1B, OSBP-related protein 1, oxysterol binding protein-like 1A, oxysterol binding protein-like 1B, oxysterol-binding protein-related protein 1 variant 2

Entrez Gene IDs

114876 (Human); 64291 (Mouse); 259221 (Rat)

Gene Symbol

OSBPL1A

UniProt

Q9BXW6 (Human)

Additional ORP1 Products

Product Documents for ORP1 Antibody

Certificate of Analysis

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Product Specific Notices for ORP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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