ORP150/HSP12A Antibody (201) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89890

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 201 expressed in HEK293

Format

Azide and BSA Free
View all ORP150/HSP12A Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human ORP150/HSP12A (Accession#: NP_001124463.1; Met 695-Leu 994).

Specificity

No cross-reactivity in ELISA with: Human Fetuin A

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ORP150/HSP12A Antibody (201) - Azide and BSA Free

Western Blot: ORP150/HSP12A Antibody (201) [NBP2-89890]

Western Blot: ORP150/HSP12A Antibody (201) [NBP2-89890]

Western Blot: ORP150/HSP12A Antibody (201) [NBP2-89890] - Lane A: 293T Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:111 kDa Observed band size:170 kDa
Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (201) [NBP2-89890]

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (201) [NBP2-89890]

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (201) [NBP2-89890] - Staining of human HSP12A in human cirrhosis with rabbit monoclonal antibody (1:200).
Immunoprecipitation: ORP150/HSP12A Antibody (201) [NBP2-89890]

Immunoprecipitation: ORP150/HSP12A Antibody (201) [NBP2-89890]

Immunoprecipitation: ORP150/HSP12A Antibody (201) [NBP2-89890] - Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate 2 uL anti-HSP12A rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-HSP12A rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 111 kDaObserved band size: 111 kDa
ORP150/HSP12A Antibody (201) - Azide and BSA Free

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (201) [NBP2-89890] -

Immunohistochemistry-Paraffin: ORP150/HSP12A Antibody (201) [NBP2-89890] - Staining of human ORP150/HSP12A in human colon carcinoma with NBP2-89890 (1:200).
ORP150/HSP12A Antibody (201) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody (201) [NBP2-89890] -

Immunocytochemistry/Immunofluorescence: ORP150/HSP12A Antibody (201) [NBP2-89890] - Staining of ORP150 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-89890 antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.

Applications for ORP150/HSP12A Antibody (201) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ORP150/HSP12A

HYOU1 is encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment is found at the 5' end of the first exon, and is involved in stress-dependent induction. A transcript that is produced from a downstream start site is preferentially induced by hypoxia, resulting in the accumulation of this protein in the endoplasmic reticulum (ER). The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol.

Long Name

Oxygen Regulated Protein, 150 kDa

Alternate Names

HSP12A, HYOU1

Gene Symbol

HYOU1

Additional ORP150/HSP12A Products

Product Documents for ORP150/HSP12A Antibody (201) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ORP150/HSP12A Antibody (201) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Heat Shock Proteins (HSPs)

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Protocols

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