Osteocalcin Antibody (2T1J7)

Novus Biologicals | Catalog # NBP3-16081

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2T1J7 expressed in HEK293
View all Osteocalcin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein of human Osteocalcin.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Osteocalcin Antibody (2T1J7)

Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081]

Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081]

Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081] - Analysis of extracts of various cell lines, using Osteocalcin antibody (NBP3-16081) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081]

Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081]

Western Blot: Osteocalcin Antibody (2T1J7) [NBP3-16081] - Analysis of extracts of various cell lines, using Osteocalcin antibody (NBP3-16081) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Osteocalcin Antibody (2T1J7)

Immunocytochemistry/ Immunofluorescence: Osteocalcin Antibody (2T1J7) [NBP3-16081] -

Immunocytochemistry/ Immunofluorescence: Osteocalcin Antibody (2T1J7) [NBP3-16081] - Immunofluorescence analysis of NIH/3T3 cells using Osteocalcin Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Osteocalcin Antibody (2T1J7)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Osteocalcin

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium

Long Name

Bone gamma-Carboxyglutamate [gla] Protein

Alternate Names

BGLAP, BGP, OCN

Gene Symbol

BGLAP

Additional Osteocalcin Products

Product Documents for Osteocalcin Antibody (2T1J7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Osteocalcin Antibody (2T1J7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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