Osteopontin/OPN Antibody (001) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89443

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 001 expressed in HEK293

Format

Azide and BSA Free
View all Osteopontin/OPN Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Osteopontin/OPN (Accession#: NP_001035147.1; Met 1-Asn 314).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Osteopontin/OPN Antibody (001) - Azide and BSA Free

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443] - Staining of human SPP1 in human hepatoma with rabbit monoclonal antibody (1:2000)
Flow Cytometry: Osteopontin/OPN Antibody (001) [NBP2-89443]

Flow Cytometry: Osteopontin/OPN Antibody (001) [NBP2-89443]

Flow Cytometry: Osteopontin/OPN Antibody (001) [NBP2-89443] - Analysis of purified anti-human Spp1 on U937 cells. U937 cells were treated and stained with Purified Rabbit anti-Spp1 (Bold line hisgram), To demonstrate specificity of staining the binding of anti-human Spp1 was blocked by the preincubation of the purified antibody with molar excess of recombinant human Spp1 (5 ug,) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody.
Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443]

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (001) [NBP2-89443] - Staining of human SPP1 in human kidney with rabbit monoclonal antibody (1:2000)

Applications for Osteopontin/OPN Antibody (001) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Flow Cytometry

1:25-1:100

Immunohistochemistry-Paraffin

1:1000-1:4000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody (001) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Osteopontin/OPN Antibody (001) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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