Osteopontin/OPN Antibody (OSP/4589)

Novus Biologicals | Catalog # NBP3-48989

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Mink, Rabbit, Turkey

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Functional

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # OSP/4589
View all Osteopontin/OPN Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length rat Spp1 protein (Uniprot: P10451)

Localization

Secreted

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Theoretical MW

34.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Scientific Data Images for Osteopontin/OPN Antibody (OSP/4589)

Osteopontin/OPN Antibody (OSP/4589)

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (OSP/4589) [NBP3-48989] -

Formalin-fixed, paraffin-embedded human brain stained with Osteopontin/OPN Antibody (OSP/4589). Inset: PBS instead of primary antibody; secondary only negative control.
Osteopontin/OPN Antibody (OSP/4589)

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (OSP/4589) [NBP3-48989] -

IHC analysis of formalin-fixed, paraffin-embedded human prostate stained by Osteopontin/OPN Antibody (OSP/4589) at 2ug/ml in PBS. HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

Applications for Osteopontin/OPN Antibody (OSP/4589)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Osteopontin/OPN

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Osteopontin/OPN Antibody (OSP/4589)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Osteopontin/OPN Antibody (OSP/4589)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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