OSTF1 Antibody (9G4) - BSA Free

Novus Biologicals | Catalog # NBP2-59396

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 9G4

Format

BSA Free
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Product Specifications

Immunogen

Recombinant human OSTF1 (1-217 aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for OSTF1 Antibody (9G4) - BSA Free

Western Blot: OSTF1 Antibody (9G4)BSA Free [NBP2-59396]

Western Blot: OSTF1 Antibody (9G4)BSA Free [NBP2-59396]

Western Blot: OSTF1 Antibody (9G4) [NBP2-59396] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OSTF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HepG2 cell lysate Lane 2 : MCF7 cell lysate Lane 3 : Jurkat cell lysate Lane 4 : LnCap cell lysate
Immunocytochemistry/ Immunofluorescence: OSTF1 Antibody (9G4) - BSA Free [NBP2-59396]

Immunocytochemistry/ Immunofluorescence: OSTF1 Antibody (9G4) - BSA Free [NBP2-59396]

Immunocytochemistry/Immunofluorescence: OSTF1 Antibody (9G4) [NBP2-59396] - Analysis of OSTF1 in MCF7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human OSTF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of OSTF1 in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human OSTF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: OSTF1 Antibody (9G4) - BSA Free [NBP2-59396]

Flow Cytometry: OSTF1 Antibody (9G4) - BSA Free [NBP2-59396]

Flow Cytometry: OSTF1 Antibody (9G4) [NBP2-59396] - Analysis of OSTF1 in HeLa cell line, staining at 2-5ug for 1x10 6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).
Western Blot: OSTF1 Antibody (9G4)BSA Free [NBP2-59396]

Western Blot: OSTF1 Antibody (9G4)BSA Free [NBP2-59396]

Western Blot: OSTF1 Antibody (9G4) [NBP2-59396] - Lane 1: 293T cell lysates, Lane 2: OSTF1 Transfected 293T cell lysates

Applications for OSTF1 Antibody (9G4) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1009

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OSTF1

Osteoclast-stimulating factor-1 is an intracellular protein produced by osteoclasts that indirectly induces osteoclast formation and bone resorption (Reddy et al., 1998 [PubMed 10092216]).[supplied by OMIM]

Alternate Names

FLJ20559, OSFSH3P2bA235O14.1, osteoclast stimulating factor 1, osteoclast-stimulating factor 1

Gene Symbol

OSTF1

Additional OSTF1 Products

Product Documents for OSTF1 Antibody (9G4) - BSA Free

Certificate of Analysis

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Product Specific Notices for OSTF1 Antibody (9G4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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