Otx1 Antibody (1F2) - Azide and BSA Free

Novus Biologicals | Catalog # H00005013-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F2

Format

Azide and BSA Free
View all Otx1 Primary Antibodies »

Product Specifications

Immunogen

OTX1 (NP_055377, 10 a.a. ~ 116 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YGMNGLGLAGPAMDLLHPSVGYPATPRKQRRERTTFTRSQLDVLEALFAKTRYPDIFMREEVALKINLPESRVQVWFKNRRAKCRQQQQSGSGTKSRPAKKKSSPVR

Specificity

OTX1 (1F2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Otx1 Antibody (1F2) - Azide and BSA Free

Western Blot: Otx1 Antibody (1F2) [H00005013-M01]

Western Blot: Otx1 Antibody (1F2) [H00005013-M01]

Western Blot: Otx1 Antibody (1F2) [H00005013-M01] - Analysis of OTX1 expression in transfected 293T cell line by OTX1 monoclonal antibody (M01), clone 1F2.Lane 1: OTX1 transfected lysate(37.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Otx1 Antibody (1F2) [H00005013-M01]

Immunocytochemistry/ Immunofluorescence: Otx1 Antibody (1F2) [H00005013-M01]

Immunocytochemistry/Immunofluorescence: Otx1 Antibody (1F2) [H00005013-M01] - Analysis of monoclonal antibody to OTX1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Otx1 Antibody (1F2) [H00005013-M01]

Western Blot: Otx1 Antibody (1F2) [H00005013-M01]

Western Blot: Otx1 Antibody (1F2) [H00005013-M01] - Analysis of OTX1 over-expressed 293 cell line, cotransfected with OTX1 Validated Chimera RNAi ( Cat # H00005013-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with OTX1 monoclonal antibody (M01), clone 1F2 (Cat # H00005013-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
ELISA: Otx1 Antibody (1F2) [H00005013-M01]

ELISA: Otx1 Antibody (1F2) [H00005013-M01]

ELISA: Otx1 Antibody (1F2) [H00005013-M01] - Detection limit for recombinant GST tagged OTX1 is approximately 0.03ng/ml as a capture antibody.

Applications for Otx1 Antibody (1F2) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Otx1

This gene encodes a member of the bicoid sub-family of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and may play a role in brain and sensory organ development. A similar protein in mice is required for proper brain and sensory organ development and can cause epilepsy.

Long Name

Orthodenticle Homolog 1

Alternate Names

FLJ38361, homeobox protein OTX1, MGC15736, orthodenticle (Drosophila) homolog 1, orthodenticle homeobox 1, Orthodenticle homolog 1, orthodenticle homolog 1 (Drosophila)

Entrez Gene IDs

5013 (Human)

Gene Symbol

OTX1

OMIM

600036 (Human)

UniProt

NP_055377 (Human)

Additional Otx1 Products

Product Documents for Otx1 Antibody (1F2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Otx1 Antibody (1F2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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