OX40/TNFRSF4 Antibody (Ber-ACT35) - BSA Free

Novus Biologicals | Catalog # NBP2-62202

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # Ber-ACT35

Format

BSA Free
View all OX40/TNFRSF4 Primary Antibodies »

Product Specifications

Immunogen

HTLV 1-transformed HUT-102 cells

Specificity

The mouse monoclonal antibody Ber-ACT35 (also known as ACT35) recognizes an extracellular epitope of OX40/TNFRSF4, an approximately 50 kDa type I transmembrane glycoprotein expressed on activated T cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OX40/TNFRSF4 Antibody (Ber-ACT35) - BSA Free

Immunohistochemistry-Paraffin: OX40/TNFRSF4 Antibody (Ber-ACT35) [NBP2-62202]

Immunohistochemistry-Paraffin: OX40/TNFRSF4 Antibody (Ber-ACT35) [NBP2-62202]

Immunohistochemistry-Paraffin: OX40/TNFRSF4 Antibody (Ber-ACT35) [NBP2-62202] - Human classic Hodgkin's lymphoma stained with OX40 (clone Ber-ACT35) at dilution 1:50, pH9 heat-induced epitope retrieval. Image from verified customer review.
Flow Cytometry: OX40/TNFRSF4 Antibody (Ber-ACT35) [NBP2-62202]

Flow Cytometry: OX40/TNFRSF4 Antibody (Ber-ACT35) [NBP2-62202]

Flow Cytometry: OX40/TNFRSF4 Antibody (Ber-ACT35) [NBP2-62202] - Staining of human PMA + ionomycin activated PBMC with anti-human CD134 (Ber-ACT35) PE.
Flow (Cell Surface): OX40/TNFRSF4 Antibody (Ber-ACT35) [NBP2-62202]

Flow (Cell Surface): OX40/TNFRSF4 Antibody (Ber-ACT35) [NBP2-62202]

Flow (Cell Surface): OX40/TNFRSF4 Antibody (Ber-ACT35) [NBP2-62202] - Surface staining of human PHA-activated leukocytes with anti-human CD134 (Ber-ACT35) purified, GAM APC.

Applications for OX40/TNFRSF4 Antibody (Ber-ACT35) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

3-6 ug/ml

Immunohistochemistry

5-10 ug/ml

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Reviewed Applications

Read 1 review rated 3 using NBP2-62202 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: OX40/TNFRSF4

The OX40 receptor is also known as CD134, ACT35, and tumor necrosis factor receptor superfamily member 4. This receptor is expressed on activated CD4+ and CD8+ T cells and B cells. The OX40 receptor binds to the OX40 ligand to provide a co-stimulatory signal that is independent of CD28. Blockade of OX40-OX40 ligand interactions has been shown to ameliorate experimental EAE and inflammatory bowel disease, imply that these interactions are important in the pathogenesis of some autoimmune diseases.

Alternate Names

ACT-135, ACT35 antigen, ACT35ATC35 antigen, CD134, CD134 antigen, Ly-70, OX40 cell surface antigen, OX40 homologue, OX40L receptor, OX40lymphoid activation antigene ACT35, TAX transcriptionally-activated glycoprotein 1 receptor, tax-transcriptionally activated glycoprotein 1 receptor, TNFRSF4, tumor necrosis factor receptor superfamily member 4, tumor necrosis factor receptor superfamily, member 4, Txgp1, TXGP1L, TXGP1LOX40 antigen

Gene Symbol

TNFRSF4

Additional OX40/TNFRSF4 Products

Product Documents for OX40/TNFRSF4 Antibody (Ber-ACT35) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for OX40/TNFRSF4 Antibody (Ber-ACT35) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for OX40/TNFRSF4 Antibody (Ber-ACT35) - BSA Free (1)

3 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
0%
3 Stars
100%
2 Stars
0%
1 Stars
0%

Have you used OX40/TNFRSF4 Antibody (Ber-ACT35) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • OX40/TNFRSF4 Antibody (Ber-ACT35)
    Name: Anonymous
    Application: Immunofluorescence - paraffin
    Sample Tested: Hodgkin lymphoma and Classic Hodgkin's lymphoma
    Species: Human
    Verified Customer | Posted 03/09/2019
    Human classic Hodgkin's lymphoma stained with Ber-ACT35 OX40 1:50
    pH9 heat-induced epitope retrieval
    OX40/TNFRSF4 Antibody (Ber-ACT35) - BSA Free NBP2-62202

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies