Key Product Details

Species Reactivity

Human, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunoprecipitation

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # Ber-ACT35
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Product Specifications

Immunogen

HTLV 1-transformed HUT-102 cells

Specificity

The mouse monoclonal antibody Ber-ACT35 (also known as ACT35) recognizes an extracellular epitope of OX40/TNFRSF4, an approximately 50 kDa type I transmembrane glycoprotein expressed on activated T cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for OX40/TNFRSF4 Antibody (Ber-ACT35) [Alexa Fluor® 405]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: OX40/TNFRSF4

The OX40 receptor is also known as CD134, ACT35, and tumor necrosis factor receptor superfamily member 4. This receptor is expressed on activated CD4+ and CD8+ T cells and B cells. The OX40 receptor binds to the OX40 ligand to provide a co-stimulatory signal that is independent of CD28. Blockade of OX40-OX40 ligand interactions has been shown to ameliorate experimental EAE and inflammatory bowel disease, imply that these interactions are important in the pathogenesis of some autoimmune diseases.

Alternate Names

ACT-135, ACT35 antigen, ACT35ATC35 antigen, CD134, CD134 antigen, Ly-70, OX40 cell surface antigen, OX40 homologue, OX40L receptor, OX40lymphoid activation antigene ACT35, TAX transcriptionally-activated glycoprotein 1 receptor, tax-transcriptionally activated glycoprotein 1 receptor, TNFRSF4, tumor necrosis factor receptor superfamily member 4, tumor necrosis factor receptor superfamily, member 4, Txgp1, TXGP1L, TXGP1LOX40 antigen

Gene Symbol

TNFRSF4

Additional OX40/TNFRSF4 Products

Product Documents for OX40/TNFRSF4 Antibody (Ber-ACT35) [Alexa Fluor® 405]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for OX40/TNFRSF4 Antibody (Ber-ACT35) [Alexa Fluor® 405]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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