p120-catenin Antibody (M354)

Name: p120-catenin Antibody (M354)
Brand: Novus Biologicals
SKU: NBP3-23202
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23202

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # M354

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Product Specifications

Immunogen

Clone (M354) was generated from a peptide that includes amino acids from the central region of human p120-catenin. This peptide sequence is highly conserved in rat and mouse p120-catenin. [UniProt# O60716]

Specificity

The antibody detects a 110 kDa protein corresponding to the molecular mass of p120-catenin on SDS-PAGE immunoblots of human A431 and HUVEC cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for p120-catenin Antibody (M354)

Immunocytochemistry/Immunofluorescence: p120-catenin Antibody (M354) [NBP3-23202] -

Immunocytochemistry/Immunofluorescence: p120-catenin Antibody (M354) [NBP3-23202] - Immunocytochemical labeling of p120-catenin in untreated (Top) or pervanadate-treated (bottom) A431 cells. The cells were labeled with mouse monoclonal p120-catenin (a.a. 275-285), p120-catenin (Tyr-228), p120-catenin (Tyr-280), or p120-catenin (Tyr-302) antibodies. The antibodies were detected using donkey anti-mouse secondary antibodies conjugated to Cy3.

Western Blot: p120-catenin Antibody (M354) [NBP3-23202] -

Western Blot: p120-catenin Antibody (M354) [NBP3-23202] - Western blot analysis of p120-catenin phosphorylation in A431 cells stimulated with calyculin A (100 nM) for 30 min. (lanes 1 & 3) then the blot was treated with lambda phosphatase (lanes 2 & 4). The blots were probed with either mouse monoclonal anti-p120-catenin (a.a. 275-285) (lanes 1 & 2) or rabbit polyclonal anti-p120-catenin (Thr-916) (lanes 3 & 4).

Applications for p120-catenin Antibody (M354)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunoprecipitation

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: p120-catenin

The alpha, beta, delta, and gamma -catenins are proteins that bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. alpha-catenin, a 102 kDa protein, interacts with E-cadherin associated protein, and also associates with other members of the cadherin family, such as N-cadherin and P-cadherin. The 92 kDa beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. beta-catenin also complexes with the tumor suppressor protein APC. delta-catenin interacts with Presenilin 1 and is expressed in the brain. The gene encoding delta-catenin maps to human chromosome 5p15.2. A hemizygous loss of the gene encoding delta-catenin leads to the mental retardation associated with Cri-du-Chat syndrome. gamma-catenin, also known as plakoglobin, is an 80-88 kDa protein that binds alpha-catenin and N-cadherin. In addition, the transmembrane phosphatase PTPm associates with catenin/cadherin complexes and may regulate complex signaling.

Alternate Names

Cadherin-associated Src substrate, CAS, catenin (cadherin-associated protein), delta 1, catenin delta-1, CTNND, KIAA0384P120CAS, p120, p120 catenin, p120(cas), p120(CTN), p120cas, p120ctn

Gene Symbol

CTNND1

Additional p120-catenin Products

Product Documents for p120-catenin Antibody (M354)

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Certificate of Analysis

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Product Specific Notices for p120-catenin Antibody (M354)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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