Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 651308
View all p15INK4b/CDKN2B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p15INK4b/CDKN2B
Arg2-Asp138
Accession # P42772

Specificity

Detects human p15INK4b/CDKN2B in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human (rh) p16INK4a and no cross-reactivity with rhp18INK4c or rhp19INK4d is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for p15INK4b/CDKN2B Antibody (651308) [FITC]

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: p15INK4b/CDKN2B

The normal progression of cells through the cell cycle is under the control of the cyclin dependent protein kinases Cdk4 and Cdk6 which are subject to inhibition by the mitotic inhibitory protein, p16. An isolated member of the p16 family has been designated p15. p15 expression is upregulated approximately 30-fold in TGFbeta-treated human keratinocytes, suggesting that p15 may act as an effector of TGFbeta-mediated cell cycle arrest. The gene encoding p15 has been mapped to chromosome 9p21 at a position adjacent to the p16 gene at a site of frequent chromosomal abnormality in human tumors. It has been suggested that p15 may function as an effector of TGFbeta-mediated cell cycle arrest through inhibition of Cdk4 and Cdk6 kinases.

Long Name

p15 Cyclin Dependent Kinase 4 Inhibitor 2B

Alternate Names

CDKN2B, MTS2

Gene Symbol

CDKN2B

Additional p15INK4b/CDKN2B Products

Product Documents for p15INK4b/CDKN2B Antibody (651308) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for p15INK4b/CDKN2B Antibody (651308) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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