Detects human p15INK4b/CDKN2B in direct ELISAs and Western blots.
In Western blots, approximately 50%
cross-reactivity with recombinant human (rh) p16INK4a and no cross-reactivity
with rhp18INK4c or rhp19INK4d is observed.
Monoclonal Mouse IgG2B Clone # 651308
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human p15INK4b/CDKN2B Arg2-Asp138 Accession # P42772
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human p15INK4b/CDKN2B by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human p15INK4b/CDKN2B Monoclonal Antibody (Catalog # MAB6798) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p15INK4b/CDKN2B at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
p15INK4b/CDKN2B in HeLa Human Cell Line. p15INK4b/CDKN2B was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human p15INK4b/CDKN2B Monoclonal Antibody (Catalog # MAB6798) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
p15INK4B is an approximately 15 kDa member of the p16INK4 family. Human p15INK4B is 138 amino acids (aa) in length and contains four ankyrin repeats, a common structural sequence found in members of the INK4 family. The first 50 aa of p15INK4B share 44% sequence identity with p16INK4. Following this is an 81 aa region sharing 97% sequence identity, after which the two proteins diverge. Human p15INK4B shares 83% aa sequence identity with mouse and rat p15INK4B. Functionally, p15INK4B interacts strongly with CDK4 and CDK6 and is a potent inhibitor. p15INK4B can potentially induce cell cycle arrest by acting as an effector of TGF-beta.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.