Key Product Details

Species Reactivity

Validated:

Human

Cited:

Xenograft

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 651308
View all p15INK4b/CDKN2B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p15INK4b/CDKN2B
Arg2-Asp138
Accession # P42772

Specificity

Detects human p15INK4b/CDKN2B in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human (rh) p16INK4a and no cross-reactivity with rhp18INK4c or rhp19INK4d is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human p15INK4b/CDKN2B Antibody

Detection of Human p15INK4b/CDKN2B antibody by Western Blot.

Detection of Human p15INK4b/CDKN2B by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human p15INK4b/CDKN2B Monoclonal Antibody (Catalog # MAB6798) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p15INK4b/CDKN2B at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

p15INK4b/CDKN2B antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

p15INK4b/CDKN2B in HeLa Human Cell Line.

p15INK4b/CDKN2B was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human p15INK4b/CDKN2B Monoclonal Antibody (Catalog # MAB6798) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human p15INK4b/CDKN2B Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p15INK4b/CDKN2B

p15INK4B is an approximately 15 kDa member of the p16INK4 family. Human p15INK4B is 138 amino acids (aa) in length and contains four ankyrin repeats, a common structural sequence found in members of the INK4 family. The first 50 aa of p15INK4B share 44% sequence identity with p16INK4. Following this is an 81 aa region sharing 97% sequence identity, after which the two proteins diverge. Human p15INK4B shares 83% aa sequence identity with mouse and rat p15INK4B. Functionally, p15INK4B interacts strongly with CDK4 and CDK6 and is a potent inhibitor. p15INK4B can potentially induce cell cycle arrest by acting as an effector of TGF-beta.

Long Name

p15 Cyclin Dependent Kinase 4 Inhibitor 2B

Alternate Names

CDKN2B, MTS2

Entrez Gene IDs

1030 (Human); 12579 (Mouse); 25164 (Rat)

Gene Symbol

CDKN2B

UniProt

P42772

Additional p15INK4b/CDKN2B Products

Product Documents for Human p15INK4b/CDKN2B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human p15INK4b/CDKN2B Antibody

For research use only

Citations for Human p15INK4b/CDKN2B Antibody

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Protocols

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