Human p15INK4b/CDKN2B Antibody

• Species Reactivity
  Human
• Specificity
  Detects human p15INK4b/CDKN2B in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human (rh) p16INK4a and no cross-reactivity with rhp18INK4c or rhp19INK4d is observed.
• Source
  Monoclonal Mouse IgG_2B Clone # 651308
• Purification
  Protein A or G purified from hybridoma culture supernatant
• Immunogen
  E. coli-derived recombinant human p15INK4b/CDKN2B
  Arg2-Asp138
  Accession # P42772
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  1 µg/mL
  See below
• Immunocytochemistry
  3-25 µg/mL
  See below

Western Blot click image to view larger

Detection of Human p15INK4b/CDKN2B by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human p15INK4b/CDKN2B Monoclonal Antibody (Catalog # MAB6798) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p15INK4b/CDKN2B at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry click image to view larger

p15INK4b/CDKN2B in HeLa Human Cell Line. p15INK4b/CDKN2B was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human p15INK4b/CDKN2B Monoclonal Antibody (Catalog # MAB6798) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reconstitution
  Sterile PBS to a final concentration of 0.5 mg/mL.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• Long Name:
  p15 Cyclin Dependent Kinase 4 Inhibitor 2B

• Entrez Gene IDs:
  1030 (Human); 12579 (Mouse); 25164 (Rat)

• Alternate Names:
  CDK4I; CDKN2B; cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4); cyclin-dependent kinases 4 and 6 binding protein; INK4BCDK4B inhibitor; MTS2; MTS-2; MTS2CDK inhibitory protein; Multiple tumor suppressor 2; p14_CDK inhibitor; p14_INK4B; p14-INK4b; p15 CDK inhibitor; p15_INK4B; P15cyclin-dependent kinase 4 inhibitor B; p15INK4b; p15-INK4b; TP15

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