p16INK4a/CDKN2A Antibody (1D7D2A1) - BSA Free

Novus Biologicals | Catalog # NBP2-37736

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1D7D2A1

Format

BSA Free
View all p16INK4a / CDKN2A Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human p16INK4a/CDKN2A (AA: 1-156) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

16.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p16INK4a/CDKN2A Antibody (1D7D2A1) - BSA Free

Western Blot Analysis of p16INK4a/CDKN2A in Multiple Cell Lysates

Western Blot Analysis of p16INK4a/CDKN2A in Multiple Cell Lysates

Western blot analysis using CDKN2A mouse mAb against Hela (1), HepG2 (2) and Hek293 (3) cell lysate.
Immunohistochemical Staining of p16INK4a/CDKN2A in Paraffin Embedded Endometrial Cancer

Immunohistochemical Staining of p16INK4a/CDKN2A in Paraffin Embedded Endometrial Cancer

Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using CDKN2A mouse mAb with DAB staining.
Flow Cytometry of HeLa Cells Stained with p16INK4a/CDKN2A Antibody

Flow Cytometry of HeLa Cells Stained with p16INK4a/CDKN2A Antibody

Flow cytometric analysis of Hela cells using CDKN2A mouse mAb (green) and negative control (red).
Western Blot Detection of Recombinant p16INK4a/CDKN2A

Western Blot Detection of Recombinant p16INK4a/CDKN2A

Western blot analysis using CDKN2A mAb against human CDKN2A (AA: 1-156) recombinant protein. (Expected MW is 19 kDa)
Western Blot Analysis of p16INK4a/CDKN2A in HEK293 Cell Lysates

Western Blot Analysis of p16INK4a/CDKN2A in HEK293 Cell Lysates

Western blot analysis using CDKN2A mAb against HEK293 (1) and CDKN2A (AA: 1-156)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical Staining of p16INK4a/CDKN2A in Paraffin Embedded Esophageal Cancer

Immunohistochemical Staining of p16INK4a/CDKN2A in Paraffin Embedded Esophageal Cancer

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CDKN2A mouse mAb with DAB staining.
Detection of Serially Diluted p16INK4a/CDKN2A in ELISA

Detection of Serially Diluted p16INK4a/CDKN2A in ELISA

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for p16INK4a/CDKN2A Antibody (1D7D2A1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p16INK4a / CDKN2A

CDKN2A/p16INK4a is a gene that codes for a widely expressed protein with four isoforms, measuring 156, 105, 116, and 167 amino acids in length with weights of approximately 17, 11, 12, and 18 kDa respectively. CDKN2A/p16INK4a functions as a negative regulator of the proliferation of cells, which then allows them to interact with cyclins D and to phosphorylate the retinoblastoma protein. Current studies are being done on several diseases and disorders related to this gene including Li-Fraumeni syndrome, malignant peripheral nerve sheath tumor, blastic plasmacytoid dendritic cell, acth-secreting pituitary adenoma, recessive dystrophic epidermolysis bullosa, marginal zone b-cell lymphoma, adult astrocytic tumor, melanoma, and squamous cell carcinoma. CDKN2A/p16INK4a has also been shown to have interactions with MDM2, ZNF420, EGR1, MYCN, and CDK6 in pathways such as the cell cycle, p53 signaling, mitochondrial apoptosis, HIF-1 Alpha, CRHR, pancreatic cancer, and glioma pathways.

Long Name

p16 Cyclin Dependent Kinase 4 Inhibitor 2A

Alternate Names

ARF, CDK4I, CDKN2A, INK4a, MLM, p14ARF, p16, p19ARF

Gene Symbol

CDKN2A

Additional p16INK4a / CDKN2A Products

Product Documents for p16INK4a/CDKN2A Antibody (1D7D2A1) - BSA Free

Certificate of Analysis

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Product Specific Notices for p16INK4a/CDKN2A Antibody (1D7D2A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for p16INK4a/CDKN2A Antibody (1D7D2A1) - BSA Free

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Protocols

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