p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free

Novus Biologicals | Catalog # NBP3-45125

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 30859-S17

Format

BSA Free
View all p16INK4a / CDKN2A Primary Antibodies »

Product Specifications

Immunogen

BALB/c mice were immunized with recombinant p16INK4a/CDKN2A (Uniprot#: P42771)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Scientific Data Images for p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free

p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free

Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free [NBP3-45125] -

Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free [NBP3-45125] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, permeabilized with 0.15% Triton, on coverslips stained with NBP3-45125 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-45125, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.

Applications for p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The binding specificity and affinity of this antibody for p16 was determined by ELISA. Western blotting experiments suggested that this antibody is capable of recognizing p16 and tumor cells expressing p16 molecules like Hela cells.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p16INK4a / CDKN2A

CDKN2A/p16INK4a is a gene that codes for a widely expressed protein with four isoforms, measuring 156, 105, 116, and 167 amino acids in length with weights of approximately 17, 11, 12, and 18 kDa respectively. CDKN2A/p16INK4a functions as a negative regulator of the proliferation of cells, which then allows them to interact with cyclins D and to phosphorylate the retinoblastoma protein. Current studies are being done on several diseases and disorders related to this gene including Li-Fraumeni syndrome, malignant peripheral nerve sheath tumor, blastic plasmacytoid dendritic cell, acth-secreting pituitary adenoma, recessive dystrophic epidermolysis bullosa, marginal zone b-cell lymphoma, adult astrocytic tumor, melanoma, and squamous cell carcinoma. CDKN2A/p16INK4a has also been shown to have interactions with MDM2, ZNF420, EGR1, MYCN, and CDK6 in pathways such as the cell cycle, p53 signaling, mitochondrial apoptosis, HIF-1 Alpha, CRHR, pancreatic cancer, and glioma pathways.

Long Name

p16 Cyclin Dependent Kinase 4 Inhibitor 2A

Alternate Names

ARF, CDK4I, CDKN2A, INK4a, MLM, p14ARF, p16, p19ARF

Gene Symbol

CDKN2A

Additional p16INK4a / CDKN2A Products

Product Documents for p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free

Certificate of Analysis

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Product Specific Notices for p16INK4a/CDKN2A Antibody (30859-S17) - Chimeric - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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