p18INK4c/CDKN2C Antibody (1Q10Q4)

Novus Biologicals | Catalog # NBP3-16695

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1Q10Q4 expressed in HEK293
View all p18INK4c/CDKN2C Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human p18INK4c/CDKN2C (P42773). MAEPWGNELASAAARGDLEQLTSLLQNNVNVNAQNGFGRTALQVMKLGNPEIARRLLLRGANPDLKDRTGFAVIHDAARAGFLDTLQTLLEFQADVNIEDNEGNLPLHLAAKEGHLRVVE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p18INK4c/CDKN2C Antibody (1Q10Q4)

Western Blot: p18INK4c/CDKN2C Antibody (1Q10Q4) [NBP3-16695]

Western Blot: p18INK4c/CDKN2C Antibody (1Q10Q4) [NBP3-16695]

Western Blot: p18INK4c/CDKN2C Antibody (1Q10Q4) [NBP3-16695] - Western blot analysis of extracts of various cell lines, using p18INK4c/CDKN2C Rabbit mAb (NBP3-16695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: p18INK4c/CDKN2C Antibody (1Q10Q4) [NBP3-16695]

Immunocytochemistry/ Immunofluorescence: p18INK4c/CDKN2C Antibody (1Q10Q4) [NBP3-16695]

Immunocytochemistry/Immunofluorescence: p18INK4c/CDKN2C Antibody (1Q10Q4) [NBP3-16695] - Immunofluorescence analysis of HeLa cells using p18INK4c/CDKN2C Rabbit mAb (NBP3-16695) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
p18INK4c/CDKN2C Antibody (1Q10Q4)

Western Blot: p18INK4c/CDKN2C Antibody (1Q10Q4) [NBP3-16695] -

Western Blot: p18INK4c/CDKN2C Antibody (1Q10Q4) [NBP3-16695] - Western blot analysis of extracts from wild type(WT) and CDKN2C knockout (KO) 293T cells, using CDKN2C antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.
p18INK4c/CDKN2C Antibody (1Q10Q4)

Western Blot: p18INK4c/CDKN2C Antibody (1Q10Q4) [NBP3-16695] -

Western Blot: p18INK4c/CDKN2C Antibody (1Q10Q4) [NBP3-16695] - Western blot analysis of extracts of HepG2 cells, using CDKN2C antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.

Applications for p18INK4c/CDKN2C Antibody (1Q10Q4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p18INK4c/CDKN2C

p18 INK4c is a specific inhibitor of cdk4 / cdk6 (interacts strongly with CDK6 and weakly with CDK4), and is expressed during G1 to S transition in the eukaryotic cell division cycle. It is maximally induced as cells enter S phase. Highest expression levels found in skeletal muscle. p18 INK4c is also found in pancreas and heart.

Long Name

p18 Cyclin Dependent Kinase 4 Inhibitor 2C

Alternate Names

CDKN2C, p18-INK6

Gene Symbol

CDKN2C

Additional p18INK4c/CDKN2C Products

Product Documents for p18INK4c/CDKN2C Antibody (1Q10Q4)

Certificate of Analysis

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Product Specific Notices for p18INK4c/CDKN2C Antibody (1Q10Q4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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