p21/CIP1/CDKN1A Antibody (1032) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43697

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Human, Mouse

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1032

Format

Azide and BSA Free
View all p21/CIP1/CDKN1A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human p21/CIP1/CDKN1A. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p21/CIP1/CDKN1A Antibody (1032) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697]

Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697]

Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 stained by p21 Cip1 antibody [1032] diluted at 1:500.Blue: Hoechst 33342 staining. Scale bar= 10 um.
Immunoprecipitation: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697]

Immunoprecipitation: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697]

Immunoprecipitation: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] - Analysis of HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr A. 30 ug HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr B. Control with 4 ug of preimmune Mouse IgG C. Immunoprecipitation of CDKN1A protein by 4 ug p21 Cip1 antibody [1032] 15 % SDS-PAGE The immunoprecipitated CDKN1A protein was detected by p21 Cip1 antibody [1032] diluted at 1:1000. [EasyBlot anti-mouse IgG was used as a secondary reagent]
p21/CIP1/CDKN1A Antibody (1032) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] -

p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDK4 protein stained by CDK4 antibody diluted at 1:1000.
Red: p21 Cip1 protein stained by p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:500.
Blue: Hoechst 33342 staining.
p21/CIP1/CDKN1A Antibody (1032) - Azide and BSA Free

Western Blot: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] -

Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
p21/CIP1/CDKN1A Antibody (1032) - Azide and BSA Free

Western Blot: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] -

Untreated (-) and treated (+) HCT-116 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
p21/CIP1/CDKN1A Antibody (1032) - Azide and BSA Free

Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] -

p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
p21 Cip1 stained by p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
p21/CIP1/CDKN1A Antibody (1032) - Azide and BSA Free

Flow Cytometry: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] -

p21 Cip1 antibody [GT1032] (NBP2-43697) detects p21 Cip1 by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: p21 Cip1 antibody [GT1032] (NBP2-43697) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.
p21/CIP1/CDKN1A Antibody (1032) - Azide and BSA Free

Western Blot: p21/CIP1/CDKN1A Antibody (1032) [NBP2-43697] -

Untreated (-) and treated (+) HCT 116 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (NBP2-43697) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for p21/CIP1/CDKN1A Antibody (1032) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p21/CIP1/CDKN1A

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Long Name

p21 Cyclin Dependent Kinase 4 Inhibitor 1A

Alternate Names

CDKN1A, CIP1

Gene Symbol

CDKN1A

Additional p21/CIP1/CDKN1A Products

Product Documents for p21/CIP1/CDKN1A Antibody (1032) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for p21/CIP1/CDKN1A Antibody (1032) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Cell Cycle

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Protocols

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